Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge

Autor(es)

Zhang, Dunhua ^[1] ; Moreira, Gabriel S. A. ^[2] ; Shoemaker, Craig ^[1] ; Newton, Joseph C. ^[3] ; Xu, De-Hai ^[1]

Afiliação do(s) autor(es)

^[1] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 - USA

^[2] Univ Sao Paulo, Lab Parasitol, Coll Anim Sci & Food Engn, Ave Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[3] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 - USA

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 363, n. 9 MAY 2016.
• Citações Web of Science: 10

Resumo

The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus. Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a time course was detected and quantified using real-time polymerase chain reaction. The results showed that 77.8% of fish died within 48 h post challenge with mean day to death of 1.5 days. At 2 h post challenge, vAh (inferred from genomic DNA copies or genome equivalents) was detected in all external and internal tissues sampled. Gill had the highest vAh cells at 1 h post challenge. Spleen harbored the most vAh cells among internal organs at 4 h post challenge. The tissues/organs with most vAh cells detected at 8 h post challenge were adipose fin, blood, intestine, kidney and skin, while liver showed the highest vAh cells at 24 h post challenge. These results suggest that vAh was able to rapidly proliferate and spread, following wound infection, through the fish blood circulation system and cause mortality within 8-24 h. (AU)

• Processo FAPESP: 15/01822-0 - Comparação da expressão de genes imunológicos do peixe Ictalurus punctatus mantido em diferentes temperaturas após infecção com o parasita Ichthyophthirius multifiliis
• Beneficiário: Gabriel Sassarão Alves Moreira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado