Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

Autor(es)

Adabbo, Mayna ^[1] ; Paolillo, Fernanda Rossi ^[2] ; Bossini, Paulo Sergio ^[3] ; Rodrigues, Natalia Camargo ^[4] ; Bagnato, Vanderlei Salvador ^[2] ; Parizotto, Nivaldo Antonio ^[3]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Biotechnol Program, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, Opt Grp, Av Trabalhador Saocarlense, 400 Ctr, BR-13560970 Sao Carlos, SP - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Phys Therapy, Electrothermophototherapy Lab, Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Biomech Med & Rehabil Locomotor Syst, Av Trabalhador Saocarlense, 400 Ctr, BR-13560970 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photomedicine and Laser Surgery; v. 34, n. 5, p. 187-193, MAY 2016.
• Citações Web of Science: 3

Resumo

Objective: The aim of this study was to analyze the effects of low-level laser therapy (LLLT) when associated with treadmill training on the recovery of skeletal muscle, during two periods of rest after muscle injury in rats. Background data: Because of photostimulation, LLLT has been presented as an alternative for accelerating the tissue healing process. Materials and methods: Forty rats were divided into two groups (A and B) containing four subgroups each: GC (Control Group)-cryolesion untreated; EG (Exercise Group)-cryolesion treated with physical exercise; LG (Laser Group)-cryolesion treated with laser; ELG (Exercise and Laser Group)-cryolesion treated with laser and physical exercise. The right tibialis anterior (TA) of the middle belly was injured by a cooling iron bar (cryoinjury). Group A remained at rest for 3 days, whereas Group B remained at rest for 7 days. The laser parameters utilized were 780nm with 15mW average optical power and spot size of 0.04 cm(2) applied during 10sec, leading to 0.152J and 3.8J/cm(2). Treadmill training with and without laser application was performed during 5 days, with each session lasting for 12min at a velocity of 17m/min. Subsequently, the TA muscle was removed for a histological and morphometric analysis. Results: The damaged area was significantly smaller for the ELG at both periods of rest, 3 and 7 days, respectively (4.4 +/- 0.42% and 3.5 +/- 0.14%, p<0.05), when compared with the LG (18.6 +/- 0.64% and 7.5 +/- 0.13%), the EG (21 +/- 0.26% and 8.7 +/- 0.32%), and the CG (23.9 +/- 0.37% and 21.4 +/- 0.38%). In addition, the number of blood vessels were significantly higher for the ELG at both periods of rest, 3 and 7 days, respectively (71.2 +/- 13.51 and 104.5 +/- 11.78, p<0.05), when compared with the LG (60.6 +/- 11.25 and 93.5 +/- 16.87), the EG (51.6 +/- 7.3 and 93.8 +/- 15.1) and the CG (34.4 +/- 2.54 and 65.7 +/- 14.1). Conclusions: The LLLT applied before the physical exercise on the treadmill stimulated the angiogenesis and accelerated the process of muscle recovery. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/14001-9 - Espectroscopia de fluorescência em dentes de ratas ovariectomizadas para o diagnóstico da osteoporose
• Beneficiário: Fernanda Rossi Paolillo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado