Genetic analysis across life stages of Metrodorea nigra (Rutaceae) in a population located in an urban landscape of Southeastern Brazil using a new set of microsatellite markers

Autor(es)

Alzate-Marin, Ana Lilia ^{[1, 2]} ; Bonifacio-Anacleto, Fernando ^[1] ; de Moraes Filho, Romulo M. ^{[1, 2]} ; Machado, Gabriel Pinheiro ^[1] ; Nazareno, Alison Goncalves ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Genet Vegetal, Dept Genet, Fac Med Ribeirao Preto, Bloco B, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Fac Med Ribeirao Preto FMRP USP RP, Dept Genet, Programa Posgrad, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Dept Bot, Inst Biociencias, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN JOURNAL OF BOTANY; v. 39, n. 2, p. 795-799, JUN 2016.
• Citações Web of Science: 0

Resumo

Metrodorea nigra A. St.-Hil. (Rutaceae) is a submontane tropical tree species that develops preferably in the shade and innermost preserved regions of the semideciduous forests of Brazil; as a result, its presence can indicate good levels of conservation. Therefore, it is important to have a set of molecular markers to facilitate genetic studies on this species of tree. The goal of this study was to perform genetic analyses of three generations of one population of M. nigra located in an urban landscape (Ribeiracao Preto, Sao Paulo state) using a new set of microsatellite molecular markers (i.e., SSR-simple sequence repeat). A genomic library was developed from M. nigra, and 11 putative molecular markers were isolated and characterized using samples from different life stages. Seven SSR markers amplified and showed polymorphism across 61 M. nigra samples. A total of 83 alleles were detected, with an average of 11.85 alleles per locus. The genetic diversity index (He) across M. nigra generations was high and ranged from a mean of 0.804 (saplings) to 0.827 (adults). These results show the resilience of M. nigra population located in Bosque Municipal de Ribeiracao Preto-SP, Brazil, to adverse effects of fragmentation, and can serve as a base for conservation programs. Furthermore, more than 50 % of the primers were also amplified for the congeneric tree species Metrodorea stipularis. Overall, the new marker set developed here will be useful for studies of population genetics of M. nigra and other key tree species from the Atlantic Rainforest of Brazil. (AU)

• Processo FAPESP: 13/18633-0 - IDENTIFICAÇÃO DE MARCADORES ESPECIE-ESPECÍFICOS DE DUAS ESPÉCIES FLORESTAIS DO GENERO Anadenanthera
• Beneficiário: Fernando Bonifácio Anacleto
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/01265-8 - Estudos genéticos em fina escala de duas espécies arbóreas comuns em remanescentes da Floresta Estacional Semidecidual no interior de SP
• Beneficiário: Ana Lilia Alzate Marin
• Modalidade de apoio: Auxílio à Pesquisa - Regular