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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pressure dependence of backbone chemical shifts in the model peptides Ac-Gly-Gly-Xxx-Ala-NH2

Texto completo
Autor(es):
Erlach, Markus Beck [1, 2] ; Koehler, Joerg [1, 2] ; Crusca, Jr., Edson [3] ; Kremer, Werner [1, 2] ; Munte, Claudia E. [3] ; Kalbitzer, Hans Robert [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Regensburg, Inst Biophys & Phys Biochem, D-93040 Regensburg - Germany
[2] Univ Regensburg, Ctr Magnet Resonance Chem & Biomed, D-93040 Regensburg - Germany
[3] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Biomolecular NMR; v. 65, n. 2, p. 65-77, JUN 2016.
Citações Web of Science: 7
Resumo

For a better understanding of nuclear magnetic resonance (NMR) detected pressure responses of folded as well as unstructured proteins the availability of data from well-defined model systems are indispensable. In this work we report the pressure dependence of chemical shifts of the backbone atoms H-1(alpha), C-13(alpha) and C-13' in the protected tetrapeptides Ac-Gly-Gly-Xxx-Ala-NH2 (Xxx one of the 20 canonical amino acids). Contrary to expectation the chemical shifts of these nuclei have a nonlinear dependence on pressure in the range from 0.1 to 200 MPa. The polynomial pressure coefficients B (1) and B (2) are dependent on the type of amino acid studied. The coefficients of a given nucleus show significant linear correlations suggesting that the NMR observable pressure effects in the different amino acids have at least partly the same physical cause. In line with this observation the magnitude of the second order coefficients of nuclei being direct neighbors in the chemical structure are also weakly correlated. (AU)

Processo FAPESP: 10/01362-5 - Interação de proteínas com modelos de microdomínios lipídicos e membranas biológicas
Beneficiário:Claudia Elisabeth Munte
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 10/12953-4 - Aplicação da RMN na elucidação estrutural dos domínios C-terminais das septinas SEPT2 e SEPT4 e interação com a proteína alfa-sinucleína
Beneficiário:Edson Crusca Junior
Linha de fomento: Bolsas no Brasil - Pós-Doutorado