Magnetic super-hydrophilic carbon nanotubes/graphene oxide composite as nanocarriers of mesenchymal stem cells: Insights into the time and dose dependences

Autor(es)

Granato, Alessandro E. C. ^{[1, 2]} ; Rodrigues, Bruno V. M. ^[1] ; Rodrigues-Junior, Dorival M. ^[3] ; Marciano, Fernanda R. ^[1] ; Lobo, Anderson O. ^[1] ; Porcionatto, Marimelia A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Biomed Nanotechnol NANOBIO, Av Shishima Hifumi 2911, BR-12224000 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Escola Paulista Med, Neurobiol Lab, Dept Biochem, Rua Pedro Toledo 669, BR-04039032 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Lab Mol Canc Biol, Rua Pedro Toledo 669, BR-04039032 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 67, p. 694-701, OCT 1 2016.
• Citações Web of Science: 4

Resumo

Among nanostructured materials, multi-walled carbon nanotubes (MWCNT) have demonstrated great potential for biomedical applications in recent years. After oxygen plasma etching, we can obtain super-hydrophilic MWCNT that contain graphene oxide (GO) at their tips. This material exhibits good dispersion in biological systems due to the presence of polar groups and its excellent magnetic, properties due to metal particle residues from the catalyst that often remain trapped in its walls and tips. Here, we show for the first time a careful biological investigation using magnetic superhydrophilic MWCNT/GO (GCN composites). The objective of this study was to investigate the application of GCN for the in vitro immobilization of mesenchymal stem cells. Our ultimate goal was to develop a system to deliver mesenchymal stem cells to different tissues and organs. We show here that mesenchymal stem cells were able to internalize GCN with a consequent migration when subjected to a magnetic field. The cytotoxicity of GCN was time- and dose-dependent. We also observed that GCN internalization caused changes in the gene expression of the proteins involved in cell adhesion and migration, such as integrins, laminins, and the chemokine CXCL12, as well as its receptor CXCR4. These results suggest that GCN represents a potential new platform for mesenchymal stem cell immobilization at injury sites. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/00652-5 - Estudo dos mecanismos moleculares da migração, sobrevivência e diferenciação de células-tronco neurais
• Beneficiário: Marimélia Aparecida Porcionatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/08523-8 - Desenvolvimento de um nanosensor fluorescente a partir da eletrofiação de PVA e quantum dots de grafeno: aplicação na detecção de biomarcadores de Alzheimer
• Beneficiário: Bruno Vinícius Manzolli Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores