Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Magnetic super-hydrophilic carbon nanotubes/graphene oxide composite as nanocarriers of mesenchymal stem cells: Insights into the time and dose dependences

Full text
Author(s):
Granato, Alessandro E. C. [1, 2] ; Rodrigues, Bruno V. M. [1] ; Rodrigues-Junior, Dorival M. [3] ; Marciano, Fernanda R. [1] ; Lobo, Anderson O. [1] ; Porcionatto, Marimelia A. [2]
Total Authors: 6
Affiliation:
[1] Univ Vale Paraiba UNIVAP, Inst Res & Dev IP&D, Lab Biomed Nanotechnol NANOBIO, Av Shishima Hifumi 2911, BR-12224000 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Neurobiol Lab, Dept Biochem, Rua Pedro Toledo 669, BR-04039032 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Lab Mol Canc Biol, Rua Pedro Toledo 669, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 67, p. 694-701, OCT 1 2016.
Web of Science Citations: 4
Abstract

Among nanostructured materials, multi-walled carbon nanotubes (MWCNT) have demonstrated great potential for biomedical applications in recent years. After oxygen plasma etching, we can obtain super-hydrophilic MWCNT that contain graphene oxide (GO) at their tips. This material exhibits good dispersion in biological systems due to the presence of polar groups and its excellent magnetic, properties due to metal particle residues from the catalyst that often remain trapped in its walls and tips. Here, we show for the first time a careful biological investigation using magnetic superhydrophilic MWCNT/GO (GCN composites). The objective of this study was to investigate the application of GCN for the in vitro immobilization of mesenchymal stem cells. Our ultimate goal was to develop a system to deliver mesenchymal stem cells to different tissues and organs. We show here that mesenchymal stem cells were able to internalize GCN with a consequent migration when subjected to a magnetic field. The cytotoxicity of GCN was time- and dose-dependent. We also observed that GCN internalization caused changes in the gene expression of the proteins involved in cell adhesion and migration, such as integrins, laminins, and the chemokine CXCL12, as well as its receptor CXCR4. These results suggest that GCN represents a potential new platform for mesenchymal stem cell immobilization at injury sites. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation
Grantee:Marimélia Aparecida Porcionatto
Support Opportunities: Regular Research Grants
FAPESP's process: 15/08523-8 - Development of a fluorescent nanosensor from the electrospinning of PVA and graphene quantum dots: application in the detection of Alzheimer's biomarkers
Grantee:Bruno Vinícius Manzolli Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants