Regulation of neural stem cells by choroid plexus cells population

Autor(es)

Roballo, Kelly C. S. ^[1] ; Goncalves, Natalia J. N. ^[1] ; Pieri, Naira C. G. ^[2] ; Souza, Aline F. ^[2] ; Andrade, Andre F. C. ^[3] ; Ambrosio, Carlos E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience Letters; v. 626, p. 35-41, JUL 28 2016.
• Citações Web of Science: 3

Resumo

The choroid plexus is a tissue on the central nervous system responsible for producing cerebrospinal fluid, maintaining homeostasis and neural stem cells support; though, all of its functions still unclear. This study aimed to demonstrate the niches of choroid plexus cells for a better understanding of the cell types and functions, using the porcine as the animal model. The collected material was analyzed by histology, immunohistochemistry, and cell culture. The cell culture was characterizated by immunocytochemistry and flow cytometry. Our results showed OCT-4, TUBIII, Nestin, CD45, CD73, CD90 positive expression and GFAP, CD105 negative expression, also methylene blue histological staining confirmed the presence of telocytes cells. We realized that the choroid plexus is a unique and incomparable tissue with different niches of cells as pluripotent, hematopoietic, neuronal progenitors and telocyte cells, which provide its complexity, differentiated functionality and responsibility on brain balance and neural stem cells regulation. (C) 2016 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 15/17897-9 - Comunicação exossomal na transdiferenciação de células-tronco em co-cultivo com células neuronais
• Beneficiário: Kelly Cristine Santos Roballo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/07673-0 - Caracterização das células oriundas do plexo coroide utilizando o suíno como modelo animal
• Beneficiário: Kelly Cristine Santos Roballo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica