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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Metformin treatment modulates the tumour-induced wasting effects in muscle protein metabolism minimising the cachexia in tumour-bearing rats

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Autor(es):
Oliveira, Andre G. [1] ; Gomes-Marcondes, Maria Cristina C. [1]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Struct & Funct Biol, Rua Monteiro Lobato 255, CP 6109, BR-13083862 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: BMC CANCER; v. 16, JUL 7 2016.
Citações Web of Science: 17
Resumo

Background: Cancer-cachexia state frequently induces both fat and protein wasting, leading to death. In this way, the knowledge of the mechanism of drugs and their side effects can be a new feature to treat and to have success, contributing to a better life quality for these patients. Metformin is an oral drug used in type 2 diabetes mellitus, showing inhibitory effect on proliferation in some neoplastic cells. For this reason, we evaluated its modulatory effect on Walker-256 tumour evolution and also on protein metabolism in gastrocnemius muscle and body composition. Methods: Wistar rats received or not tumour implant and metformin treatment and were distributed into four groups, as followed: control (C), Walker 256 tumour-bearing (W), metformin-treated (M) and tumour-bearing treated with metformin (WM). Animals were weighed three times a week, and after cachexia state has been detected, the rats were euthanised and muscle and tumour excised and analysed by biochemical and molecular assays. Results: Tumour growth promoted some deleterious effects on chemical body composition, increasing water and decreasing fat percentage, and reducing lean body mass. In muscle tissue, tumour led to a decreased protein synthesis and an increased proteolysis, showing the higher activity of the ubiquitin-proteasome pathway. On the other hand, the metformin treatment likely minimised the tumour-induced wasting state; in this way, this treatment ameliorated chemical body composition, reduced the higher activities of proteolytic enzymes and decreased the protein waste. Conclusion: Metformin treatment not only decreases the tumour growth but also improves the protein metabolism in gastrocnemius muscle in tumour-bearing rats. (AU)

Processo FAPESP: 13/16115-1 - Estudo dos mecanismos bioquímicos e moleculares da caquexia: avaliação dos efeitos do fator Walker (FW) e da leucina em cultura celular de miotúbulos (células C2C12)
Beneficiário:Maria Cristina Cintra Gomes Marcondes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/07737-0 - Avaliação da associação entre o tratamento com metformina e suplementação nutricional com leucina no metabolismo protéico de ratos portadores do Tumor de Walker 256
Beneficiário:André Gustavo de Oliveira
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 10/00209-9 - Nutrição e câncer estudo das alterações metabólicas e moleculares do metabolismo protéico no câncer-caquexia, moduladas pela suplementação nutricional com leucina, no crescimento, desenvolvimento e gravidez
Beneficiário:Maria Cristina Cintra Gomes Marcondes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/13334-7 - Nutrição e câncer: avaliação do perfil metabolômico, proteômico e das vias de sinalização celular muscular, placentária e fetal e do tecido tumoral sob influência dos hormônios da gravidez em ratas
Beneficiário:Maria Cristina Cintra Gomes Marcondes
Linha de fomento: Auxílio à Pesquisa - Regular