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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Acute exercise restores insulin clearance in diet-induced obese mice

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Autor(es):
Kurauti, Mirian A. [1] ; Costa-Junior, Jose M. [1] ; Ferreira, Sandra M. [1] ; dos Santos, Gustavo J. [1] ; Protzek, Andre O. P. [1] ; Nardelli, Tarlliza R. [1] ; de Rezende, Luiz F. [1] ; Boschero, Antonio C. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Endocrinology; v. 229, n. 3, p. 221-232, JUN 2016.
Citações Web of Science: 11
Resumo

The aim of this study was to investigate the insulin clearance in diet-induced obese (DIO) mice submitted to acute endurance exercise (3 h of treadmill exercise at 60-70% VO(2)max). Glucose-stimulated insulin secretion in isolated islets; ipGTT; ipITT; ipPTT; in vivo insulin clearance; protein expression in liver, skeletal muscle, and adipose tissue (insulin degrading enzyme (IDE), insulin receptor subunit beta (IR beta), phospho-Akt (p-Akt) and phospho-AMPK (p-AMPK)), and the activity of IDE in the liver and skeletal muscle were accessed. In DIO mice, acute exercise reduced fasting glycemia and insulinemia, improved glucose and insulin tolerance, reduced hepatic glucose production, and increased p-Akt protein levels in liver and skeletal muscle and p-AMPK protein levels in skeletal muscle. In addition, insulin secretion was reduced, whereas insulin clearance and the expression of IDE and IR beta were increased in liver and skeletal muscle. Finally, IDE activity was increased only in skeletal muscle. In conclusion, we propose that the increased insulin clearance and IDE expression and activity, primarily, in skeletal muscle, constitute an additional mechanism, whereby physical exercise reduces insulinemia in DIO mice. (AU)

Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
Beneficiário:Licio Augusto Velloso
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 13/21283-0 - Função da IL-6 no clearance de insulina em camundongos obesos submetidos ao exercício físico agudo
Beneficiário:Mirian Ayumi Kurauti
Linha de fomento: Bolsas no Brasil - Mestrado