Acute exercise restores insulin clearance in diet-induced obese mice

Autor(es)

Kurauti, Mirian A. ^[1] ; Costa-Junior, Jose M. ^[1] ; Ferreira, Sandra M. ^[1] ; dos Santos, Gustavo J. ^[1] ; Protzek, Andre O. P. ^[1] ; Nardelli, Tarlliza R. ^[1] ; de Rezende, Luiz F. ^[1] ; Boschero, Antonio C. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Endocrinology; v. 229, n. 3, p. 221-232, JUN 2016.
• Citações Web of Science: 11

Resumo

The aim of this study was to investigate the insulin clearance in diet-induced obese (DIO) mice submitted to acute endurance exercise (3 h of treadmill exercise at 60-70% VO(2)max). Glucose-stimulated insulin secretion in isolated islets; ipGTT; ipITT; ipPTT; in vivo insulin clearance; protein expression in liver, skeletal muscle, and adipose tissue (insulin degrading enzyme (IDE), insulin receptor subunit beta (IR beta), phospho-Akt (p-Akt) and phospho-AMPK (p-AMPK)), and the activity of IDE in the liver and skeletal muscle were accessed. In DIO mice, acute exercise reduced fasting glycemia and insulinemia, improved glucose and insulin tolerance, reduced hepatic glucose production, and increased p-Akt protein levels in liver and skeletal muscle and p-AMPK protein levels in skeletal muscle. In addition, insulin secretion was reduced, whereas insulin clearance and the expression of IDE and IR beta were increased in liver and skeletal muscle. Finally, IDE activity was increased only in skeletal muscle. In conclusion, we propose that the increased insulin clearance and IDE expression and activity, primarily, in skeletal muscle, constitute an additional mechanism, whereby physical exercise reduces insulinemia in DIO mice. (AU)

• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/21283-0 - Função da IL-6 no clearance de insulina em camundongos obesos submetidos ao exercício físico agudo
• Beneficiário: Mirian Ayumi Kurauti
• Linha de fomento: Bolsas no Brasil - Mestrado