Comparison of liposomal and 2-hydroxypropyl-beta-cyclodextrin-lidocaine on cell viability and inflammatory response in human keratinocytes and gingival fibroblasts

Autor(es)

Nunes Ferreira, Luiz Eduardo ^[1] ; Muniz, Bruno Vilela ^[1] ; dos Santos, Cleiton Pita ^[1] ; Volpato, Maria Cristina ^[1] ; de Paula, Eneida ^[1] ; Groppo, Francisco Carlos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, Limeira Ave 901, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 68, n. 6, p. 791-802, JUN 2016.
• Citações Web of Science: 2

Resumo

Objectives The aim of this study was to observe the effect multilamellar liposomes (MLV) and 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) in the in-vitro effects of lidocaine in cell viability, pro-inflammatory cytokines and prostaglandin E-2 release of both human keratinocytes (HaCaT) and gingival fibroblasts (HGF) cells. Methods HaCaT and HGF cells were exposed to lidocaine 100-1 mu M in plain, MLV and HP-beta-CD formulations for 6 h or 24 h. The formulation effects in cell viability were measured by XTT assay and by fluorescent labelling. Cytokines (IL-8, IL-6 and TNF-alpha) and PGE(2) release were quantified by ELISA. Key findings MLV and HP-beta-CD formulations did not affect the HaCaT viability, which was significantly decreased by plain lidocaine after 24 h of exposure. Both drug carriers increased all cytokines released by HGF after 24-h exposure, and none of the carriers was able to reduce the PGE(2) release induced by lidocaine. Conclusion The effect of drug carrier in the lidocaine effects was dependent on the cell type, concentration and time of exposure. MLV and HP-beta-CD showed benefits in improving cell viability; however, both of them showed a tendency to increase cytokine release when compared to the plain solution. (AU)

• Processo FAPESP: 12/07310-2 - Efeitos modulatórios de anestésicos locais associados a carreadores sobre a produção de mediadores inflamatórios e viabilidade celular em células epiteliais orais e fibroblastos gengivais humanos
• Beneficiário: Francisco Carlos Groppo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/12666-8 - Efeitos dos anestésicos locais associados a carreadores na modulação de mediadores inflamatórios, viabilidade celular e indução da apoptose
• Beneficiário: Luiz Eduardo Nunes Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto