High prevalence of HBV/A1 subgenotype in native south Americans may be explained by recent economic developments in the Amazon

Autor(es)

Godoy, Bibiane A. ^[1] ; Gomes-Gouvea, Michele S. ^[2] ; Zagonel-Oliveira, Marcelo ^{[1, 3]} ; Alvarado-Mora, Monica V. ^[2] ; Salzano, Francisco M. ^[1] ; Pinho, Joao R. R. ^{[4, 2]} ; Fagundes, Nelson J. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Dept Genet, Caixa Postal 15053, BR-91501970 Porto Alegre, RS - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Trop Gastroenterol & Hepatol Joao Alves de Qu, Inst Trop Med, Dept Gastroenterol, Sao Paulo, SP - Brazil

^[3] Univ Vale Rio dos Sinos, VIZLab Adv Visualizat Lab, Sao Leopoldo, RS - Brazil

^[4] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 43, p. 354-363, SEP 2016.
• Citações Web of Science: 1

Resumo

Native American populations present the highest prevalence of Hepatitis B Virus (HBV) infection in the Americas, which may be associated to severe disease outcomes. Ten HBV genotypes (A-J) have been described, displaying a remarkable geographic structure, which most likely reflects historic patterns of human migrations. In this study, we characterize the HBV strains circulating in a historical sample of Native South Americans to characterize the historical viral dynamics in this population. The sample consisted of 1070 individuals belonging to 38 populations collected between 1965 and 1997. Presence of HBV DNA was checked by quantitative real-time PCR, and determination of HBV genotypes and subgenotypes was performed through sequencing and phylogenetic analysis of a fragment including part of HBsAg and Pol coding regions (S/Pol). A Bayesian Skyline Plot analysis was performed to compare the viral population dynamics of HBV/A1 strains found in Native Americans and in the general Brazilian population. A total of 109 individuals were positive for HBV DNA (similar to 10%), and 70 samples were successfully sequenced and genotyped. Subgenotype A1 (HBV/A1), related to African populations and the African slave trade, was the most prevalent (66-94%). The Skyline Plot analysis showed a marked population expansion of HBV/A1 in Native Americans occurring more recently (1945-1965) than in the general Brazilian population. Our results suggest that historic processes that contributed to formation of HBV/A1 circulating in Native American are related with more recent migratory waves towards the Amazon basin, which generated a different viral dynamics in this region. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/53946-3 - EMU: aquisição de analisador genético de DNA para o sequenciamento automático de DNA
• Beneficiário: João Renato Rebello Pinho
• Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários