Nanowire Arrays as Cell Force Sensors To Investigate Adhesin-Enhanced Holdfast of Single Cell Bacteria and Biofilm Stability

Autor(es):Mostrar menos -

Sahoo, Prasana K. ^[1] ; Janissen, Richard ^{[1, 2]} ; Monteiro, Moniellen P. ^[1] ; Cavalli, Alessandro ^[3] ; Murillo, Duber M. ^[1] ; Merfa, Marcus V. ^[4] ; Cesar, Carlos L. ^[5] ; Carvalho, Hernandes F. ^[6] ; de Souza, Alessandra A. ^[4] ; Bakkers, Erik P. A. M. ^[3] ; Cotta, Monica A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Sao Paulo - Brazil

^[2] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands

^[3] Eindhoven Univ Technol, Dept Appl Phys, NL-5600 MB Eindhoven - Netherlands

^[4] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Sao Paulo - Brazil

^[5] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Sao Paulo - Brazil

^[6] Univ Estadual Campinas, Inst Biol, Struct & Funct Biol Dept, BR-13083865 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Nano Letters; v. 16, n. 7, p. 4656-4664, JUL 2016.
• Citações Web of Science: 22

Resumo

Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesincoated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues. (AU)

• Processo FAPESP: 13/02300-1 - Nanofios semicondutores: mecanismos de formação e aplicação em biossensores
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51748-7 - Análise estrutural e química de biofilmes de Xylella fastidiosa
• Beneficiário: Mônica Alonso Cotta
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57906-3 - Instituto Nacional de Fotônica Aplicada à Biologia Celular - INFABIC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático