| Full text | |
| Author(s): Show less - |
Sahoo, Prasana K.
[1]
;
Janissen, Richard
[1, 2]
;
Monteiro, Moniellen P.
[1]
;
Cavalli, Alessandro
[3]
;
Murillo, Duber M.
[1]
;
Merfa, Marcus V.
[4]
;
Cesar, Carlos L.
[5]
;
Carvalho, Hernandes F.
[6]
;
de Souza, Alessandra A.
[4]
;
Bakkers, Erik P. A. M.
[3]
;
Cotta, Monica A.
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Sao Paulo - Brazil
[2] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands
[3] Eindhoven Univ Technol, Dept Appl Phys, NL-5600 MB Eindhoven - Netherlands
[4] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Sao Paulo - Brazil
[5] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Sao Paulo - Brazil
[6] Univ Estadual Campinas, Inst Biol, Struct & Funct Biol Dept, BR-13083865 Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Nano Letters; v. 16, n. 7, p. 4656-4664, JUL 2016. |
| Web of Science Citations: | 22 |
| Abstract | |
Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesincoated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues. (AU) | |
| FAPESP's process: | 13/02300-1 - Semiconductor nanowires: formation mechanisms and biosensing applications |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms |
| Grantee: | Mônica Alonso Cotta |
| Support Opportunities: | Regular Research Grants |