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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Babesia bovis and Babesia bigemina infection levels estimated by qPCR in Angus cattle from an endemic area of Sao Paulo state, Brazil

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Autor(es):
Giglioti, R. ; Oliveira, H. N. ; Santana, C. H. ; Ibelli, A. M. G. ; Neo, T. A. ; Bilhassi, T. B. ; Rabelo, M. D. ; Machado, R. Z. ; Brito, L. G. ; Oliveira, M. C. S.
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: TICKS AND TICK-BORNE DISEASES; v. 7, n. 5, p. 657-662, 2016.
Citações Web of Science: 5
Resumo

The levels of infection by Babesia bovis and Babesia bigemina were estimated by absolute quantification through the quantitative PCR technique (qPCR). Fifty-one contemporaneous Angus cattle were evaluated on two occasions. The number of standard female Rhipicephalus microplus ticks present on the left side of the body was counted and blood samples were drawn from the tail vein into tubes containing the anticoagulant EDTA. The blood samples were submitted to DNA extraction and used to quantify the number of copies (NC) of DNA from B. bovis and B. bigemina by qPCR. The data on tick count and number of DNA copies were transformed for normalization and analyzed by a mixed model method. A multivariate model with repeated measures of the same animal, including the effects of collection, parasite species and their interaction, was used. The repeatability values were obtained from the matrix of (co)variances and were expressed for each species. The correlations between the counts of different species on the same animal, in the same collection or different collections, were also estimated. The results showed the qPCR could distinguish the two between infection by the two Babesia species. Infection levels by B. bovis and B. bigemina were detected in 100% and 98% of the animals, respectively. Significant differences were found (P < 0.05) between the NC of the two Babesia species, B. bovis 1.49 +/- 0.07 vs. B. bigemina 0.82 +/- 0.06. Low repeatabilities were found for the counts of R. microplus and NC of B. bovis and B. bigemina: 0.05, 0.10 and 0.02, respectively. The correlations between R. microplus count and NC Of B. bovis and B. bigemina were both very near zero. However, an association was observed between the NC of the two species, with a correlation coefficient of 0.30 for measures from the same collection. The absence of associations between the quantity of DNA from B. bovis and B. bigemina and the tick counts suggests that the variation of parasitemia by the hemoparasites did not depend on the tick infestation levels at the moment of each collection. The repeatability values estimated indicate that under the study conditions, the variations in the tick infestation levels and of parasitemia by B. bovis and B. bigemina depend more on factors related to each collection than on intrinsic factors of the animal. (C) 2016 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 12/00067-5 - Estudo quantitativo da infecção por Babesia bovis e Babesia bigemina em bovinos de corte de diferentes grupos genéticos
Beneficiário:Henrique Nunes de Oliveira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/23833-2 - Estudo quantitativo da infecção por Babesia bovis em bovinos de corte de diferentes grupos genéticos
Beneficiário:Rodrigo Giglioti
Modalidade de apoio: Bolsas no Brasil - Doutorado