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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Allele- and parent-of-origin-specific effects on expression of the KCNJ11 gene: A candidate for meat tenderness in cattle

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de Souza, M. M. ; Niciura, S. C. M. ; Tizioto, P. C. ; Ibelli, A. M. G. ; Gasparin, G. ; Rocha, M. I. P. ; Bressani, F. A. ; Malago-, Jr., W. ; Diniz, W. J. S. ; de Oliveira, P. S. N. ; Lima, A. O. ; Mudadu, M. A. ; Barioni Junior, W. ; Coutinho, L. L. ; Regitano, L. C. A.
Número total de Autores: 15
Tipo de documento: Artigo Científico
Fonte: Genetics and Molecular Research; v. 15, n. 3 2016.
Citações Web of Science: 1
Resumo

In contrast to the Mendelian inheritance model, parental alleles can contribute unequally to gene expression, which may result in phenotypic variance among individuals and bias in the predicted additive effect of molecular markers associated with production traits. Given the need to understand the effects of allelic variation and parent-of-origin effects on the expression of genes with a commercial interest in cattle, we analyzed the expression of KCNJ11 (potassium inwardly rectifying channel, subfamily J, member 11), which was previously described as a functional candidate gene for meat tenderness. Allele-specific and parent-of-origin-dependent expression of this gene were assessed in bovine muscle using the rs379610823 single nucleotide polymorphism as a reference. Biallelic expression was observed; however, the T allele was expressed at significantly higher levels than the C allele. Furthermore, increased expression of KCNJ11 was found in animals harboring the maternal T allele. This study is the first to describe the differential allelic expression of bovine KCNJ11. Our findings are important for understanding the mechanisms that underlie the pattern of KCNJ11 expression and its potential impact on the phenotypic variation of meat tenderness in Nelore beef cattle. This reinforces the need for further investigation of allelic-and parent-of-origin expression deviation in genetic markers eligible for the selection of target traits. (AU)

Processo FAPESP: 08/03916-8 - Efeito de polimorfismos e de origem parental sobre a diferenciação celular in vitro, a expressão e a epigenética em genes de interesse comercial em bovinos de corte
Beneficiário:Simone Cristina Méo Niciura
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
Beneficiário:Luciana Correia de Almeida Regitano
Linha de fomento: Auxílio à Pesquisa - Temático