Mechanisms underlying heterologous skin scaffold-mediated tissue remodeling

Autor(es)

Mimura, Kallyne K. O. ; Moraes, Andreia R. ; Miranda, Aline C. ; Greco, Rebecca ; Ansari, Tahera ; Sibbons, Paul ; Greco, Karin V. ; Oliani, Sonia M.

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, OCT 11 2016.
• Citações Web of Science: 10

Resumo

Biocompatibility of two newly developed porcine skin scaffolds was assessed after 3, 14, 21 and 90 days of implantation in rats. Both scaffolds showed absence of cells, preservation of ECM and mechanical properties comparable to non-decellularised skin before implantation. Host cell infiltration was much prominent on both scaffolds when compared to Permacol (surgical control). At day 3, the grafts were surrounded by polymorphonuclear cells, which were replaced by a notable number of IL-6-positive cells at day 14. Simultaneously, the number of pro-inflammatory M1-macrophage was enhanced. Interestingly, a predominant pro-remodeling M2 response, with newly formed vessels, myofibroblasts activation and a shift on the type of collagen expression was sequentially delayed (around 21 days). The gene expression of some trophic factors involved in tissue remodeling was congruent with the cellular events. Our findings suggested that the responsiveness of macrophages after non-crosslinked skin scaffolds implantation seemed to intimately affect various cell responses and molecular events; and this range of mutually reinforcing actions was predictive of a positive tissue remodeling that was essential for the long-standing success of the implants. Furthermore, our study indicates that non-crosslinked biologic scaffold implantation is biocompatible to the host tissue and somehow underlying molecular events involved in tissue repair. (AU)

• Processo FAPESP: 12/13041-4 - Avaliação de transplantes heterólogos de pele usando matrizes acelulares obtidas de pele suína e ação da proteína anexina A1 como agente anti-inflamatório/imunossupressor
• Beneficiário: Kallyne Kioko Oliveira Mimura
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21603-2 - Bioengenharia tecidual: avaliar os transplantes heterólogos de pele usando matrizes acelulares suínas e a proteína anexina A1 como potencial recurso terapêutico nos processos regenerativo e cicatricial
• Beneficiário: Sonia Maria Oliani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/18557-4 - Estudo dos processos inflamatório e angiogênico nos implantes heterólogos de ratos usando matrizes acelulares de derme suína
• Beneficiário: Andréia Rodrigues de Moraes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica