A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis

Autor(es)

Ribeiro Barros, Luiza Saldanha ; Carrao, Daniel Blascke ; Costa Queiroz, Regina Helena ; Moraes de Oliveira, Anderson Rodrigo ; de Gaitani, Cristiane Masetto

• Tipo de documento: Artigo Científico
• Fonte: ELECTROPHORESIS; v. 37, n. 20, p. 2678-2684, OCT 2016.
• Citações Web of Science: 2

Resumo

A three phase hollow fiber liquid-phasemicroextraction technique combined with capillary electrophoresis was developed to quantify lamotrigine (LTG) in plasma samples. The analyte was extracted from 4.0 mL of a basic donor phase (composed of 0.5 mL of plasma and 3.5 mL of sodium phosphate solution pH 9.0) through a supported liquid membrane composed of 1-octanol immobilized in the pores of the hollow fiber, and to an acidic acceptor phase (hydrochloric acid solution pH 4.0) placed in the lumen of the fiber. The extraction was carried out for 30 min at 500 rpm. The eletrophoretic analysis was carried out in 130 mmol/LMES buffer, pH 5.0 with a constant voltage of + 15 kV and 20 degrees C. Sample injections were performed for 10 s, at a pressure of 0.5 psi. The detection was performed at 214 nm for both LTG and the internal standard lidocaine. Under the optimized conditions, the method showed a limit of quantification of 1.0 mu g/mL and was linear over the plasmatic concentration range of 1.0-20.0 mu g/mL. Finally, the validated method was applied for the quantification of LTG in plasma samples of epileptic patients. (AU)

• Processo FAPESP: 13/17658-9 - Desenvolvimento e validação de métodos cromatográficos e eletroforéticos para posterior aplicação em estudos de biotransformação e metabolismo in vitro - fase 2
• Beneficiário: Anderson Rodrigo Moraes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17632-1 - Aplicações da eletroforese capilar nas áreas clínica, terapêutica e biotecnológica
• Beneficiário: Cristiane Masetto de Gaitani
• Modalidade de apoio: Auxílio à Pesquisa - Regular