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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Optical Characterization of Parathyroid Tissues

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Autor(es):
Brandao, Mariana P. ; Iwakura, Ricardo ; Honorato-Sobrinho, Abraao A. ; Haleplian, Kaique ; Ito, Amando S. ; Conti de Freitas, Luiz C. ; Bachmann, Luciano
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Applied Spectroscopy; v. 70, n. 10, p. 1709-1716, OCT 2016.
Citações Web of Science: 0
Resumo

The parathyroid glands are small and often similar to lymph nodes, fat, and thyroid tissue. These glands are difficult to identify during surgery and a biopsy of the parathyroid for identification can lead to damage of the gland. The use of static and time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and nondestructive method to assist medical evaluation of parathyroid tissues. In this study, we investigated 10 human parathyroid samples using absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. Moreover, we compared the results of time-resolved fluorescence measurements with 59 samples of thyroid tissues. The fluorescence lifetimes with emission at 340 nm were 1.09 +/- 0.10 and 4.46 +/- 0.06 ns for healthy tissue, 1.01 +/- 0.25 and 4.39 +/- 0.36 ns for benign lesions, and 0.67 +/- 0.36 and 3.92 +/- 0.72 ns for malignant lesions. The lifetimes for benign and malignant lesions were significantly different, as attested by the analysis of variance with confidence levels higher than 87%. For each class of samples (healthy, benign, and malignant) we perceived statistical differences between the thyroid and parathyroid tissue, independently. After further investigations, fluorescence methods could become a tool to identify normal and pathological parathyroid tissues and distinguish thyroid from parathyroid tissues. (AU)

Processo FAPESP: 11/07960-4 - Radiometria ótica de lasers, LEDs e lâmpadas emissoras de radiação ultravioleta
Beneficiário:Luciano Bachmann
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/26895-7 - Heterogeneidades em arranjos lipídicos e protéicos: estudos por técnicas avançadas de fluorescência
Beneficiário:Amando Siuiti Ito
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/02460-6 - Espectroscopia vibracional de tecidos biológicos: diagnóstico de lesões cancerosas e caracterização química de tecidos dentais irradiados com lasers
Beneficiário:Luciano Bachmann
Linha de fomento: Auxílio à Pesquisa - Regular