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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optical Characterization of Parathyroid Tissues

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Author(s):
Brandao, Mariana P. ; Iwakura, Ricardo ; Honorato-Sobrinho, Abraao A. ; Haleplian, Kaique ; Ito, Amando S. ; Conti de Freitas, Luiz C. ; Bachmann, Luciano
Total Authors: 7
Document type: Journal article
Source: Applied Spectroscopy; v. 70, n. 10, p. 1709-1716, OCT 2016.
Web of Science Citations: 0
Abstract

The parathyroid glands are small and often similar to lymph nodes, fat, and thyroid tissue. These glands are difficult to identify during surgery and a biopsy of the parathyroid for identification can lead to damage of the gland. The use of static and time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and nondestructive method to assist medical evaluation of parathyroid tissues. In this study, we investigated 10 human parathyroid samples using absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. Moreover, we compared the results of time-resolved fluorescence measurements with 59 samples of thyroid tissues. The fluorescence lifetimes with emission at 340 nm were 1.09 +/- 0.10 and 4.46 +/- 0.06 ns for healthy tissue, 1.01 +/- 0.25 and 4.39 +/- 0.36 ns for benign lesions, and 0.67 +/- 0.36 and 3.92 +/- 0.72 ns for malignant lesions. The lifetimes for benign and malignant lesions were significantly different, as attested by the analysis of variance with confidence levels higher than 87%. For each class of samples (healthy, benign, and malignant) we perceived statistical differences between the thyroid and parathyroid tissue, independently. After further investigations, fluorescence methods could become a tool to identify normal and pathological parathyroid tissues and distinguish thyroid from parathyroid tissues. (AU)

FAPESP's process: 11/07960-4 - Optical radiometry of lasers, LEDs and ultraviolet lamps
Grantee:Luciano Bachmann
Support Opportunities: Regular Research Grants
FAPESP's process: 14/26895-7 - Heterogeneities in lipids and proteins structures: studies by advanced fluorescence techniques
Grantee:Amando Siuiti Ito
Support Opportunities: Regular Research Grants
FAPESP's process: 12/02460-6 - Vibrational spectroscopy applied to biological tissues: cancer diagnosis and chemical characterization of laser irradiated dental tissues
Grantee:Luciano Bachmann
Support Opportunities: Regular Research Grants