Antimicrobial peptides in saliva of children with severe early childhood caries

Autor(es)

Colombo, Natalia H. ; Ribas, Lais F. F. ; Pereira, Jesse A. ; Kreling, Paula F. ; Kressirer, Christine A. ; Tanner, Anne C. R. ; Duque, Cristiane

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 69, p. 40-46, SEP 2016.
• Citações Web of Science: 12

Resumo

Objective: Controversies exist regarding the relationship between the concentrations of antimicrobial peptides (AMPs) and presence of dental caries in children. Thus, the aim of this study was to examine levels of AMPs in saliva of caries-free (CF), early childhood caries (ECC) and severe early childhood caries (S-ECC) children to determine if the levels of these salivary peptides individually or in combinations were related to caries severity and mutans streptococci levels. Design: 36 to 60 month-old children were selected to participate in this study. Children were grouped into CF group (n = 29), ECC group (n = 25) and S-ECC group (n = 29). Saliva was collected from children for microbiological analysis by culture. Salivary concentrations of cathelicidin LL-37, human beta-defensin 2 (hBD-2), human beta-defensin 3 (hBD-3) and histatin-5 (HTN-5) were determined by ELISA. Results: Salivary concentrations of AMPs did not differ among CF, ECC and S-ECC groups. Data showed positive correlations between mutans streptococci levels and salivary hBD-2 or HTN-5. Positive correlations were found between hBD-2, hBD-3, LL-37 and HTN-5. Combinations among AMPs, mainly LL-37, were positively associated with caries levels. Conclusions: Salivary concentrations of AMPs individually were not associated with the severity of early childhood caries. The stimulus of caries appears to trigger a biological response, however, with a combination of these peptides. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/12167-7 - Influência de fatores ambientais, dos níveis de microrganismos potencialmente cariogênicos e de peptídeos antimicrobianos no desenvolvimento da cárie precoce da infância
• Beneficiário: Lais Fernanda Fonseca Ribas
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/19235-5 - Papel antimicrobiano e imunológico de peptídeos catiônicos na prevenção da cárie precoce da infância e uma proposta de incorporação de fragmentos desses peptídeos em sistemas de nanopartículas como agentes anticárie
• Beneficiário: Cristiane Duque
• Modalidade de apoio: Auxílio à Pesquisa - Regular