Accessing the biocompatibility of layered double hydroxide by intramuscular implantation: histological and microcirculation evaluation

Autor(es)

Rodrigues Cunha, Vanessa Roberta ; de Souza, Rodrigo Barbosa ; Pinto da Fonseca Martins, Ana Maria Cristina Rebello ; Jun Koh, Ivan Hong ; Leopoldo Constantino, Vera Regina

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, AUG 2 2016.
• Citações Web of Science: 20

Resumo

Biocompatibility of layered double hydroxides (LDHs), also known as hydrotalcite-like materials or double metal hydroxides, was investigated by in vivo assays via intramuscular tablets implantation in rat abdominal wall. The tablets were composed by chloride ions intercalated into LDH of magnesium/aluminum (Mg2Al-Cl) and zinc/aluminum (Zn2Al-Cl). The antigenicity and tissue integration capacity of LDHs were assessed histologically after 7 and 28 days post-implantation. No fibrous capsule nearby the LDH was noticed for both materials as well any sign of inflammatory reactions. Sidestream Dark Field imaging, used to monitor in real time the microcirculation in tissues, revealed overall integrity of the microcirculatory network neighboring the tablets, with no blood flow obstruction, bleeding and/or increasing of leukocyte endothelial adhesion. After 28 days Mg2Al-Cl promoted multiple collagen invaginations (mostly collagen type-I) among its fragments while Zn2Al-Cl induced predominantly collagen type-III. This work supports previous results in the literature about LDHs compatibility with living matter, endorsing them as functional materials for biomedical applications. (AU)

• Processo FAPESP: 11/50318-1 - Desenvolvimento de compostos com interesse farmacológico ou medicinal e de sistemas para seu transporte, detecção e reconhecimento no meio biológico
• Beneficiário: Ana Maria da Costa Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/12209-9 - Carregadores inorgânicos de substâncias bioativas: desenvolvimento, caracterização, estudos de toxicidade, de liberação in vitro e de absorção in vivo após administração oral
• Beneficiário: Vanessa Roberta Rodrigues da Cunha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado