Effect of monomer blend and chlorhexidine-adding on physical, mechanical and biological properties of experimental infiltrants

Autor(es)

Inagaki, Luciana Tiemi ; Bruschi Alonso, Roberta Caroline ; Spagnolo Araujo, Giovana Albamonte ; Carualho de Souza-Junior, Eduardo Jose ; Anibal, Paula Cristina ; Hofling, Jose Francisco ; Pascon, Fernanda Miori ; Puppin-Rontani, Regina Maria

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 32, n. 12, p. E307-E313, DEC 2016.
• Citações Web of Science: 0

Resumo

Objectives. The aim of the study was to evaluate antimicrobial inhibition zone, degree of conversion (DC) and Knoop hardness (KH) of experimental infiltrants. Experimental low viscosity monomer blends were prepared and chlorhexidine diacetate salt (CHX) (0.1% or 0.2%) was added comprising the groups: G1) TEGDMA; G2) TEGDMA/0.1CHX; G3) TEGDMA/0.2CHX; G4) TEGDMA/UDMA; G5) TEGDMA/UDMA/0.1CHX; G6) TEGDMA/UDMA/0.2CHX; G7) TEGDMA/BISEMA; G8) TEGDMA/BISEMA/0.1CHX; G9) TEGDMA/BISEMA/0.2CHX. Icon (R) was used as control group. Methods. Specimens of resin blends were made (n = 9) to accomplished DC and KH. Pour plate was accomplished to evaluate antimicrobial groups' activity against Streptococcus mutans (SM) and Lactobacillus acidophilus (LA). Data obtained were submitted to two-way ANOVA and Tukey tests for blends comparisons and Dunnett's test for comparisons between experimental infiltrants and Icon (R) (p <0.05). Results. In relation to antimicrobial effect, uncured blends showed higher antibacterial activity than cured ones for the most of blends. After polymerization, G5 showed the highest inhibition zone against SM and, G3 and G6 against LA. Concerning KH, TEGDMA/UDMA-based blends showed the highest values of KH number and it was influenced by monomeric base, regardless CHX concentration. DC was not affected by monomer blend composition, neither for CHX concentration. The antimicrobial activity was affected by monomeric base, CHX concentration and polymerization. Experimental infiltrants presented similar or higher performance than Icon for the properties evaluated. Significance. White spot lesion infiltration with low viscosity monomer blends (infiltrants) is an alternative to stop initial caries lesions progression. The incorporation of an antimicrobial agent as chlorhexidine diacetate salt in infiltrants composition could enhance the performance of these materials. (C) 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/01010-1 - Análise das propriedades dos materiais infiltrantes em função da sua composição: monômeros base, diluentes e antimicrobiano
• Beneficiário: Luciana Tiemi Inagaki
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/22149-0 - Avaliação de infiltrantes experimentais quanto à solubilidade, citotoxicidade e resistência à degradação química, mecânica e biodegradação
• Beneficiário: Luciana Tiemi Inagaki
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/20254-9 - Análise das propriedades dos materiais infiltrantes em função da sua composição: monômeros base, diluentes e antimicrobiano
• Beneficiário: Regina Maria Puppin Rontani
• Modalidade de apoio: Auxílio à Pesquisa - Regular