In vitro effects of photodynamic therapy induced by chloroaluminum phthalocyanine nanoemulsion

Autor(es)

Franchi, Leonardo Pereira ; Amantino, Camila F. ; Melo, Maryanne T. ; de Lima Montaldi, Ana Paula ; Primo, Fernando L. ; Tedesco, Antonio Claudio

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 16, p. 100-105, DEC 2016.
• Citações Web of Science: 3

Resumo

Background: The photodynamic therapy (PDT) has been used to treat cancer mainly by inducing oxidative stress. Our aim was to evaluate the effect of PDT and its combination with methoxyamine (MX), a blocker of base excision repair (BER), in cells expressing high levels of the APE1 protein, which is involved in cell oxidative damage response. Methods: The HeLa and A549 cells were treated for 3 h with chloroaluminum phthalocyanine incorporated into a well-designed nanoemulsion (C1A1Pc/NE); and then irradiated by visible light (@670 nm) with doses of 0.1, 0.5 and 1.0 J/cm(2). A simultaneous combination of MX+CIA1Pc/NE was performed and then irradiated with the selected dose of 0.5 J/cm(2) . The treatments were evaluated in terms of viability, clonogenicity, DNA fragmentation, and cell death mechanism by apoptosis and/or necrosis. Results: The APE1 protein expression observed was higher in HeLa than in A549. Both cell lines exhibited substantial differences in cell cytotoxicity. The PDT decreased the clonogenicity of HeLa by inducing apoptosis (sub-G1 and annexin detection). Additionaly, the MX potentiates the PDT-effects in HeLa. Otherwise, low cytotoxicity was observed in A549 cells. Conclusion: The PDT induced apoptosis in high APE1 expressive HeLa cells, and the blockage of BER by MX increased its effects. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/11870-9 - Sensibilização de linhagens de câncer à terapia fotodinâmica por meio da inibição da proteína APE1
• Beneficiário: Leonardo Pereira Franchi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado