Identification of promising plasma immune biomarkers to differentiate active pulmonary tuberculosis

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Zambuzi, Fabiana A. ; Cardoso-Silva, Priscilla M. ; Espindola, Milena S. ; Soares, Luana S. ; Galvao-Lima, Leonardo J. ; Brauer, Veronica S. ; Gomes, Matheus S. ; Amaral, Laurence R. ; Schaller, Matthew ; Bollela, Valdes R. ; Frantz, Fabiani G.

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 88, p. 99-107, DEC 2016.
• Citações Web of Science: 8

Resumo

Although much research has been done related to biomarker discovery for tuberculosis infection, a set of biomarkers that can discriminate between active and latent TB diseases remains elusive. In the current study we correlate clinical aspects of TB disease with changes in the immune response as determined by biomarkers detected in plasma. Our study measured 18 molecules in human plasma in 17 patients with active disease (APTB), 14 individuals with latent tuberculosis infection (LTBI) and 16 uninfected controls (CTRL). We found that active tuberculosis patients have increased plasma levels of IL-6, IP-10, TNF-alpha sCD163 and sCD14. Statistical analysis of these biomarkers indicated that simultaneous measurement of sCD14 and IL-6 was able to diagnose active tuberculosis infection with 83% accuracy. We also demonstrated that TNF-alpha and sCD163 were correlated with tuberculosis severity. We showed that the simultaneous detection of both plasma sCD14 and IL-6 is a promising diagnostic approach to identify APTB, and further, measurement of TNF-alpha and sCD163 can identify the most severe cases of tuberculosis. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/12199-0 - Estudo de disfunções na resposta imune inata em pacientes HIV+: correlação com alterações imunológicas e epigenéticas
• Beneficiário: Fabiani Gai Frantz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores