| Texto completo | |
| Autor(es): |
Ochoa Cruz, Edgar Andres
;
Queiroga Cruz, Guilherme Marcello
;
Vieira, Andreia Prata
;
Van Sluys, Marie-Anne
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MOBILE DNA; v. 7, JUL 28 2016. |
| Citações Web of Science: | 0 |
| Resumo | |
Background: The genomic data available nowadays has enabled the study of repetitive sequences and their relationship to viruses. Among them, long terminal repeat retrotransposons (LTR-RTs) are the largest component of most plant genomes, the Gypsy and Copia superfamilies being the most common. Recently it has been found that Del lineage, an LTR-RT of Gypsy superfamily, has putative virus-like attachment (vl-att) sites. This signature, originally described for retroviruses, is recognized by retroviral integrase conferring specificity to the integration process. Results: Here we retrieved 26,092 putative complete LTR-RTs from 10 lineages found in 10 fully sequenced angiosperm genomes and found putative vl-att sites that are a conserved structural landmark across these genomes. Furthermore, we reveal that each plant genome has a distinguishable LTR-RT lineage amplification pattern that could be related to the vl-att sites diversity. We used these patterns to generate a specific quick-response (QR) code for each genome that could be used as a barcode of identification of plants in the future. Conclusions: The universal distribution of vl-att sites represents a new structural feature common to plant LTR-RTs and retroviruses. This is an important finding that expands the information about the structural similarity between LTR-RT and retroviruses. We speculate that the sequence diversity of vl-att sites could be important for the life cycle of retrotransposons, as it was shown for retroviruses. All the structural vl-att site signatures are strong candidates for further functional studies. Moreover, this is the first identification of specific LTR-RT content and their amplification patterns in a large dataset of LTR-RT lineages and angiosperm genomes. These distribution patterns could be used in the future with biotechnological identification purposes. (AU) | |
| Processo FAPESP: | 12/21064-4 - Construindo uma Plataforma de Biologia Sintética para a Triagem Funcional de Sequencias Promotoras em Plantas |
| Beneficiário: | Edgar Andrés Ochoa Cruz |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 08/58243-8 - Retrotransposons das famílias scMaximus, scAle e scDel: desvendando as interações com o genoma da cana-de-açúcar |
| Beneficiário: | Guilherme Marcello Queiroga Cruz |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 12/02671-7 - Homólogos a At-thi1 em cana-de-açúcar: estudo molecular e funcional |
| Beneficiário: | Andréia Prata Vieira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 08/52074-0 - Sugarcane genome sequence: plant transposable elements are active contributors to gene structure variation, regulation and function |
| Beneficiário: | Marie-Anne Van Sluys |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |