Laminin-111 peptide C16 regulates invadopodia activity of malignant cells through beta 1 integrin, Src and ERK 1/2

Autor(es)

Siqueira, Adriane S. ; Pinto, Monique P. ; Cruz, Mario C. ; Smuczek, Basilio ; Cruz, Karen S. P. ; Barbuto, Jose Alexandre M. ; Hoshino, Daisuke ; Weaver, Alissa M. ; Freitas, Vanessa M. ; Jaeger, Ruy G.

• Tipo de documento: Artigo Científico
• Fonte: ONCOTARGET; v. 7, n. 30, p. 47904-47917, JUL 26 2016.
• Citações Web of Science: 7

Resumo

Laminin peptides influence tumor behavior. In this study, we addressed whether laminin peptide C16 (KAFDITYVRLKF, gamma 1 chain) would increase invadopodia activity of cells from squamous cell carcinoma (CAL27) and fibrosarcoma (HT1080). We found that C16 stimulates invadopodia activity over time in both cell lines. Rhodamine-conjugated C16 decorates the edge of cells, suggesting a possible binding to membrane receptors. Flow cytometry showed that C16 increases activated beta 1 integrin, and beta 1 integrin miRNA-mediated depletion diminishes C16-induced invadopodia activity in both cell lines. C16 stimulates Src and ERK 1/2 phosphorylation, and ERK 1/2 inhibition decreases peptide-induced invadopodia activity. C16 also increases cortactin phosphorylation in both cells lines. Based on our findings, we propose that C16 regulates invadopodia activity over time of squamous carcinoma and fibrosarcoma cells, probably through beta 1 integrin, Src and ERK 1/2 signaling pathways. (AU)

• Processo FAPESP: 09/17336-6 - Peptídeos bioativos da laminina induzindo invadopódios em linhagem celular derivada de carcinoma epidermóide: estudo dinâmico por microscopia 4D
• Beneficiário: Adriane Sousa de Siqueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/57103-8 - Dinamica de formacao de invadopodios em linhagens tumorais tratadas por peptideos bioativos da laminina. estudo por microscopia 4d
• Beneficiário: Ruy Gastaldoni Jaeger
• Modalidade de apoio: Auxílio à Pesquisa - Regular