Human Cytomegalovirus DNA Quantification and Gene Expression in Gliomas of Different Grades

Autor(es)

Stangherlin, Lucas Matheus ; Ferreira Castro, Fabiane Lucy ; Scortegagna Medeiros, Raphael Salles ; Guerra, Juliana Mariotti ; Kimura, Lidia Midori ; Shirata, Neuza Kazumi ; Nonogaki, Suely ; dos Santos, Claudia Januario ; Carlan Silva, Maria Cristina

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 7 JUL 26 2016.
• Citações Web of Science: 6

Resumo

Gliomas are the most common type of primary brain tumors. The most aggressive type, Glioblastoma multiforme (GBM), is one of the deadliest human diseases, with an average survival at diagnosis of about 1 year. Previous evidence suggests a link between human cytomegalovirus (HCMV) and gliomas. HCMV has been shown to be present in these tumors and several viral proteins can have oncogenic properties in glioma cells. Here we have investigated the presence of HCMV DNA, RNA and proteins in fifty-two gliomas of different grades of malignancy. The UL83 viral region, the early beta 2.7 RNA and viral protein were detected in 73%, 36% and 57% by qPCR, ISH and IHC, respectively. Positivity of the viral targets and viral load was independent of tumor type or grade suggesting no correlation between viral presence and tumor progression. Our results demonstrate high prevalence of the virus in gliomas from Brazilian patients, contributing to a better understanding of the association between HCMV infection and gliomas worldwide and supporting further investigations of the virus oncomodulatory properties. (AU)

• Processo FAPESP: 13/14215-9 - Investigação do papel do citomegalovírus humano HCMV na resistência de células tumorais de glioblastoma (GBM) ao tratamento quimioterápico in vitro
• Beneficiário: Maria Cristina Carlan da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular