Molecular and serological characterization of the first Leptospira santarosai strain isolated from a dog

Autor(es)

Miotto, Bruno Alonso ; Moreno, Luisa Zanolli ; Alves Guilloux, Aline Gil ; de Sousa, Gisele Oliveira ; Loureiro, Ana Paula ; Moreno, Andrea Micke ; Lilenbaum, Walter ; Vasconcellos, Silvio Arruda ; Heinemann, Marcos Bryan ; Hagiwara, Mitika Kuribayashi

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 162, p. 1-4, OCT 2016.
• Citações Web of Science: 6

Resumo

Leptospirosis is a zoonotic disease of global importance caused by pathogenic Leptospira species. Dogs can become asymptomatically infected, acting like reservoir hosts for pathogenic Leptospira, notably Leptospira interrogans serovar Canicola. Identification of such individuals and characterization of leptospires involved in chronic infections may unravel the role of dogs in the epidemiology of particular leptospiral strains. The aim of the present work was to describe the first Leptospira santarosai strain isolated from a dog. The dog was kept in a public shelter in Sao Paulo city, Brazil, and presented asymptomatic urinary shedding detected by PCR. Prospective evaluation was performed to fully characterize its chronic carrier state. The dog did not present anti-Leptospira titles or clinical/laboratorial abnormalities during the evaluations; nevertheless long-term urinary shedding was confirmed by PCR and leptospires were recovered from two occasions. The isolated strain was molecularly characterized by partial 16S rRNA and secY gene sequencing and MLST analysis. Serogroup identification was performed using polyclonal antibodies. The strain was identified as Leptospira santarosai, serogroup Sejroe. This is the first evidence in the literature of the isolation of L santarosai in dogs. Our findings show that dogs can persistently harbor leptospires other than L interrogans. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/17136-2 - Caracterização de estirpes de Leptospira isoladas no Brasil através de tipificação por eletroforese em campo pulsado (PFGE) e análise comparativa de genomas.
• Beneficiário: Luisa Zanolli Moreno
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/14681-7 - Avaliação da leptospirúria e identificação de portadores de leptospiras patogênicas em cães mantidos em abrigos públicos ou particulares da região metropolitana de São Paulo
• Beneficiário: Mitika Kuribayashi Hagiwara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/13022-0 - Avaliação da leptospirúria e identificação de portadores de leptospiras patogênicas em cães mantidos em abrigos públicos ou particulares da Região Metropolitana de São Paulo
• Beneficiário: Bruno Alonso Miotto
• Modalidade de apoio: Bolsas no Brasil - Mestrado