Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants

Autor(es)

Oliveira, Halley C. ; Gomes, Bruna C. R. ; Pelegrino, Milena T. ; Seabra, Amedea B.

• Tipo de documento: Artigo Científico
• Fonte: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 61, p. 10-19, DEC 30 2016.
• Citações Web of Science: 14

Resumo

Nitric oxide (NO) is a signaling molecule involved in plant response to various abiotic stresses. However, the application of NO donors in agriculture is hampered by the instability of these compounds. Despite the successful uses of NO-releasing nanoparticles for biomedical purposes and the variety of nano materials developed as carrier systems of agrochemicals, the potential applications of nanocarriers for NO delivery in plants have not yet been tested. Herein, we report the synthesis and characterization of chitosan nanoparticles (CS NPs) containing the NO donor S-nitroso-mercaptosuccinic acid (S-nitrosoMSA). The efficiency of these NO-releasing NPs in mitigating the deleterious effects of salinity on maize plants was compared to that of the non-encapsulated NO donor. The NPs were synthesized through ionotropic gelation process, and mercaptosuccinic acid (MSA), the NO donor precursor, was encapsulated into CS NPs (91.07% encapsulation efficiency). Free thiol groups of MSA-CS NPs were nitrosated, leading to S-nitroso-MSA-CS NPs (NO-releasing NPs). The incorporation of S-nitroso-MSA into CS NPs allowed a sustained NO release. Treatments of salt-stressed maize plants with S-nitroso-MSA-CS NPs resulted in a higher leaf S-nitrosothiols content compared to that of free S-nitroso-MSA. Moreover, S-nitroso-MSA-CS NPs were more efficient than was the free NO donor in the amelioration of the deleterious effects of salinity in photosystem II activity, chlorophyll content and growth of maize plants because the protective action of the nanoencapsulated S-nitroso-MSA was achieved at lower dosages. Overall, these results demonstrate the positive impact of S-nitroso-MSA nanoencapsulation in increasing NO bioactivity in maize plants under salt stress. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 16/10347-6 - Nanopartículas contendo S-nitrosotióis: Síntese, caracterização, ensaios de citotoxicidade e aplicações
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/00393-8 - Nanopartículas poliméricas doadoras de óxido nítrico para aplicações tópicas
• Beneficiário: Milena Trevisan Pelegrino
• Modalidade de apoio: Bolsas no Brasil - Mestrado