| Texto completo | |
| Autor(es): |
Mendes, Juliano S.
;
Santiago, Andre S.
;
Toledo, Marcelo A. S.
;
Horta, Maria A. C.
;
de Souza, Alessandra A.
;
Tasic, Ljubica
;
de Souza, Anete P.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN MICROBIOLOGY; v. 7, DEC 26 2016. |
| Citações Web of Science: | 2 |
| Resumo | |
The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa. Here, we provide a detailed in vitro characterization of the extracellular proteins of X. fastidiosa. Based on the results, we performed a comparison with a strain J1a12, which cannot induce citrus variegated chlorosis symptoms when inoculated into citrus plants. We then extend this approach to analyze the extracellular proteins of X. fastidiosa in media supplemented with calcium. We verified increases in extracellular proteins concomitant with the days of growth and, consequently, biofilm development (330 days). Outer membrane vesicles carrying toxins were identified beginning at 10 days of growth in the 9a5c strain. In addition, a decrease in extracellular proteins in media supplemented with calcium was observed in both strains. Using mass spectrometry, 71 different proteins were identified during 30 days of X. fastidiosa biofilm development, including proteases, quorum-sensing proteins, biofilm formation proteins, hypothetical proteins, phage-related proteins, chaperones, toxins, antitoxins, and extracellular vesicle membrane components. (AU) | |
| Processo FAPESP: | 01/07533-7 - Resolução da estrutura tridimensional de proteínas relacionadas à patogenicidade de Xyllela fastidiosa |
| Beneficiário: | Anete Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/51580-4 - Caracterização funcional e molecular de proteínas potencialmente relacionadas a fitopatogenicidade da bactéria formadora de biofilme Xylella fastidiosa |
| Beneficiário: | Anete Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/50268-4 - Caracterizacao de tres fatores de transcricao pertencentes a familia lysr de xulella fastidiosa. |
| Beneficiário: | André da Silva Santiago |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |