Microbial community structure and dynamics in thermophilic composting viewed through metagenomics and metatranscriptomics

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Antunes, Luciana Principal ; Martins, Layla Farage ; Pereira, Roberta Verciano ; Thomas, Andrew Maltez ; Barbosa, Deibs ; Lemos, Leandro Nascimento ; Machado Silva, Gianluca Major ; Silva Moura, Livia Maria ; Condomitti Epamino, George Willian ; Digiampietri, Luciano Antonio ; Lombardi, Karen Cristina ; Ramos, Patricia Locosque ; Quaggio, Ronaldo Bento ; Franco de Oliveira, Julio Cezar ; Pascon, Renata Castiglioni ; da Cruz, Joao Batista ; da Silva, Aline Maria ; Setubal, Joao Carlos

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 6, DEC 12 2016.
• Citações Web of Science: 28

Resumo

Composting is a promising source of new organisms and thermostable enzymes that may be helpful in environmental management and industrial processes. Here we present results of metagenomicand metatranscriptomic-based analyses of a large composting operation in the Sao Paulo Zoo Park. This composting exhibits a sustained thermophilic profile (50 degrees C to 75 degrees C), which seems to preclude fungal activity. The main novelty of our study is the combination of time-series sampling with shotgun DNA, 16S rRNA gene amplicon, and metatranscriptome high-throughput sequencing, enabling an unprecedented detailed view of microbial community structure, dynamics, and function in this ecosystem. The time-series data showed that the turning procedure has a strong impact on the compost microbiota, restoring to a certain extent the population profile seen at the beginning of the process; and that lignocellulosic biomass deconstruction occurs synergistically and sequentially, with hemicellulose being degraded preferentially to cellulose and lignin. Moreover, our sequencing data allowed near-complete genome reconstruction of five bacterial species previously found in biomass-degrading environments and of a novel biodegrading bacterial species, likely a new genus in the order Bacillales. The data and analyses provided are a rich source for additional investigations of thermophilic composting microbiology. (AU)

• Processo FAPESP: 11/50870-6 - Estudos da diversidade microbiana no Parque Zoológico do Estado de São Paulo
• Beneficiário: João Carlos Setubal
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático