| Texto completo | |
| Autor(es): |
Domingos, Marta O.
;
Melo, Keyde C. M.
;
Neves, Irys Viana
;
Mota, Cristiane M.
;
Rui, Rita C.
;
Melo, Bruna S.
;
Lima, Raphael C.
;
Horton, Denise S. P. Q.
;
Borges, Monamaris M.
;
Franzolin, Marcia R.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF MICROBIOLOGY; v. 54, n. 11, p. 745-752, NOV 2016. |
| Citações Web of Science: | 2 |
| Resumo | |
Using clonal phylogenetic methods, it has been demonstrated that O111:H25 atypical enteropathogenic E. coli (aEPEC) strains belong to distinct clones, suggesting the possibility that their ability to interact with different hosts and abiotic surfaces can vary from one clone to another. Accordingly, the ability of O111:H25 aEPEC strains derived from human, cat and dogs to adhere to epithelial cells has been investigated, along with their ability to interact with macrophages and to form biofilms on polystyrene, a polymer used to make biomedical devices. The results demonstrated that all the strains analyzed were able to adhere to, and to form pedestals on, epithelial cells, mechanisms used by E. coli to become strongly attached to the host. The strains also show a Localized-Adherence-Like (LAL) pattern of adhesion on HEp-2 cells, a behavior associated with acute infantile diarrhea. In addition, the O111:H25 aEPEC strains derived either from human or domestic animals were able to form long filaments, a phenomenon used by some bacteria to avoid phagocytosis. O111:H25 aEPEC strains were also encountered inside vacuoles, a characteristic described for several bacterial strains as a way of protecting themselves against the environment. They were also able to induce TNF-alpha release via two routes, one dependent on TLR-4 and the other dependent on binding of Type I fimbriae. These O111:H25 strains were also able to form biofilms on polystyrene. In summary the results suggest that, regardless of their source (i.e. linked to human origin or otherwise), O111:H25 aEPEC strains carry the potential to cause human disease. (AU) | |
| Processo FAPESP: | 12/11325-5 - Avaliação da resposta imune induzida por uma vacina oral contra E. coli pertencentes aos sorogrupos O26 e O111 |
| Beneficiário: | Marta de Oliveira Domingos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |