MAPK7 Gene Controls Proliferation, Migration and Cell Invasion in Osteosarcoma

Autor(es)

Tesser-Gamba, Francine ; da Silva Lopes, Luana Joyce ; Petrilli, Antonio Sergio ; Caminada Toledo, Silvia Regina

• Tipo de documento: Artigo Científico
• Fonte: Molecular Carcinogenesis; v. 55, n. 11, p. 1700-1713, NOV 2016.
• Citações Web of Science: 11

Resumo

Osteosarcomas (OS) are the most common malignant bone tumors, and the identification of useful tumor biomarkers and target proteins is required to predict the clinical outcome of patients and therapeutic response as well as to develop novel therapeutic strategies. In our previous study, MAPK7 has been identified as a candidate oncogene, and a promising prognostic marker for OS. Sequential activation of protein kinases within the mitogen-activated protein kinase (MAPK) cascades is a common mechanism of signal transduction in many cellular processes. In this study, we investigated the behavior of MAPK7 gene in OS cell lines. Technical viability, proliferation, migration, invasion, and apoptosis were used to evaluate the function of the MAPK7 gene. We evaluated the behavior of the OS cells with MAPK7 gene silenced, not silenced, and exposed to the main chemotherapy drugs used in OS treatment. We found that silenced MAPK7 gene is effective at suppressing cell proliferation, inhibiting cell migration, and invasion. Furthermore, MAPK7 is an important activator of transcription factors and is the main expression modulator of other key genes in the MAPK pathway. In summary, our study suggests that MAPK7 might be a promising therapeutic target for OS. (C) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 11/10459-5 - Caracterização molecular e funcional dos sarcomas ósseos e de partes moles da infância e da adolescência
• Beneficiário: Silvia Regina Caminada de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/10782-8 - Estudo funcional do gene MAPK7, proposto como marcador prognóstico em osteossarcoma.
• Beneficiário: Francine Tesser Gamba
• Modalidade de apoio: Bolsas no Brasil - Doutorado