Production of Bacillus amyloliquefaciens OG and its metabolites in renewable media: valorisation for biodiesel production and p-xylene decontamination

Autor(es)

Etchegaray, Augusto ; Coutte, Francois ; Chataigne, Gabrielle ; Bechet, Max ; dos Santos, Ramon H. Z. ; Leclere, Valerie ; Jacques, Philippe

• Tipo de documento: Artigo Científico
• Fonte: Canadian Journal of Microbiology; v. 63, n. 1, p. 46-60, JAN 2017.
• Citações Web of Science: 4

Resumo

Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after analysis of the gyrA and gyrB DNA sequences. Growth in modified Landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and acetoin, which promote plant growth. Cultivation was studied in the presence of renewable carbon (as glycerol) and nitrogen (as arginine) sources. While diverse kinetics of acetoin production were observed in different media, similar yields (6-8 g.L-1) were obtained after 72 h of growth. Glycerol increased surfactin-specific production, while arginine increased the yields of surfactin and fengycin and increased biomass significantly. The specific production of fengycin increased similar to 10 times, possibly due to a connecting pathway involving arginine and ornithine. Adding value to crude extracts and biomass, both were shown to be useful, respectively, for the removal of p-xylene from contaminated water and for biodiesel production, yielding similar to 70 mg.g(-1) cells and glycerol, which could be recycled in novel media. This is the first study considering circular bioeconomy to lower the production costs of biosurfactants by valorisation of both microbial cells and their primary and secondary metabolites. (AU)

• Processo FAPESP: 13/20570-6 - Produção sustentável de biossurfactantes com aplicações para medicina e remediação ambiental
• Beneficiário: Augusto Etchegaray Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/13174-1 - Co-produção de biomassa e biossurfactantes de Bacillus subtilis reciclando alimentos, resíduos de biomassa microbiana e da síntese de biodiesel
• Beneficiário: Ramon Henrick Zorzeto dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica