A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis

Autor(es)

de Oliveira, Ricardo A. G. ; Camargo, Fiamma ; Pesquero, Naira C. ; Faria, Ronaldo Censi

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 957, p. 40-46, MAR 8 2017.
• Citações Web of Science: 36

Resumo

This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (mu PADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct mu PADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D mu PADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the mu PADs, which was photographed after 15 min using a digital camera. Both mu PADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 mu M, and 2.34 mu M for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 mu M, and 4.35 mu M. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/19890-1 - Desenvolvimento de métodos para diagnóstico de doenças utilizando partículas magnéticas e arranjo de imunossensores em sistema microfluídico descartável
• Beneficiário: Ronaldo Censi Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular