A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis

Author(s)

de Oliveira, Ricardo A. G. ; Camargo, Fiamma ; Pesquero, Naira C. ; Faria, Ronaldo Censi

• Document type: Journal article
• Source: Analytica Chimica Acta; v. 957, p. 40-46, MAR 8 2017.
• Web of Science Citations: 36

Abstract

This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (mu PADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct mu PADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D mu PADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the mu PADs, which was photographed after 15 min using a digital camera. Both mu PADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 mu M, and 2.34 mu M for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 mu M, and 4.35 mu M. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants