A fully integrated polyester-toner (PT) based microchip for genetic analyses: Development and applications

• Grant number: 15/17145-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Emanuel Carrilho
• Grantee: Emanuel Carrilho
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The production of micro total analysis (micro-TAS) has been the object of intense efforts by the scientific community in the genetic testing area. The necessity of producing a platform that performs the DNA extraction, amplification and separation is driven by the advantages associated with analysis in miniaturized platforms. Microfluidic platforms offer solutions to many problems that affect traditional genetic analyzes, both for forensic applications and for applications in clinical diagnosis, since it allows the integration of all steps involved in the analysis of DNA in a single device. Besides avoiding manipulation of the sample during analysis, the device uses small volume of sample and reagents and provides analysis in a reduced time. In search of the consolidation of this concept, this research project aims to produce polyester-toner (PT) microfluidic platforms fully integrated genetic analysis that allows the extraction, amplification and DNA separation in a single platform - a real "lab on a chip ". In addition to developing the platform is intended to develop some methodologies for application of the device. A method will be developed for detection of bacteria resistant to antibiotics from the class of beta-lactam and a method for clinical diagnosis of neglected diseases by DNA analysis (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIMENEZ, THIAGO DADALTO; BAILAO, ALEXANDRE MELO; DE ALMEIDA SOARES, CELIA MARIA; FIACCADORI, FABIOLA SOUZA; DE LIMA DIAS E SOUZA, MENIRA BORGES; DUARTE, GABRIELA R. M.. Dynamic solid-phase RNA extraction from a biological sample in a polyester-toner based microchip. ANALYTICAL METHODS, v. 9, n. 13, p. 2116-2121, APR 7 2017. (15/17145-7)

MA, HUI LING; URBACZEK, ANA CAROLINA; RIBEIRO DE SOUZA, FAYENE ZEFERINO; GOMES GARRIDO CARNEIRO LEAO, PAULO AUGUSTO; PERUSSI, JANICE RODRIGUES; CARRILHO, EMANUEL. Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility. MICROMACHINES, v. 12, n. 3, MAR 2021. (14/50867-3, 15/17145-7)

MENDES, GEOVANA M.; OLIVEIRA, KEZIA G.; BORBA, JULIANE C.; OLIVEIRA, THAIS S.; FIACCADORI, FABIOLA S.; NOGUEIRA, MAURICIO L.; BAILAO, ALEXANDRE M.; SOARES, CELIA MARIA A.; CARRILHO, EMANUEL; DUARTE, GABRIELA R. M.. Molecular Diagnostics of Dengue by Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) in Disposable Polyester-Toner Microdevices. Journal of the Brazilian Chemical Society, v. 30, n. 9, p. 1841-1849, SEP 2019. (14/50867-3, 15/17145-7)

DE OLIVEIRA, KEZIA GOMES; BORBA, JULIANE CRISTINA; BAILAO, ALEXANDRE MELO; DE ALMEIDA SOARES, CELIA MARIA; CARRILHO, EMANUEL; MENDES DUARTE, GABRIELA RODRIGUES. Loop-mediated isothermal amplification in disposable polyester-toner microdevices. Analytical Biochemistry, v. 534, p. 70-77, OCT 1 2017. (15/17145-7)