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Multidimensional chromatographic systems (Nano-HPLC and microchip) hyphenated to tandem mass spectrometry of high resolution for quantitative proteomics

Grant number: 15/16025-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2015
Effective date (End): February 29, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Emanuel Carrilho
Grantee:Weliton Pedro Batiston
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Associated scholarship(s):17/17368-1 - New analytical strategies to bottom-up and top-down proteomics, BE.EP.DR

Abstract

Among omics science, proteomics, is the one that has aroused interest of scientific research due to its great ability to create new technologies in various areas, such as the elucidation of disease, development of products (pharmacists, food and cosmetics) and beyond the understanding the constitution of living beings. However, the solution to the adequate study of one proteome depends on the set of sophisticated techniques in analytical chemistry, computational and mathematics, because of enormous complexity of biological samples. Furthermore, reliable results, requires that the method has been optimized and the expected is fast and simple analysis. In order to address these challenges, this project aims to develop two approaches to the shotgun quantitative proteomics (label-free): 1) the comprehensive multidimensional liquid chromatography coupled to tandem mass spectrometry of high resolution (MS/MS) and 2) fabrication of a comprehensive multidimensional microchip with electrospray for MS/MS. The first strategy is interesting due to the consolidated chemical principles of separation science, which enables a robust method, sensitive and with high peak capacity and orthogonality. In this study, we propose an inedited platform in the nano fluidic scale and approaches experimental and statistical, through of chemometrics, this fact can permit high separation efficiently and faster analysis. On the other hand, the second approach, is interesting because of theoretical possibility of increase of orthogonality and power resolution in miniaturization separation platforms, and principally for portability, simplicity and instrumental versatility. This approach is an innovation in multidimensional microfluidic platforms (whole integrated into the chip) coupled to MS/MS for the same type of samples and chemometric strategy.

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