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New analytical strategies to bottom-up and top-down proteomics

Grant number: 17/17368-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 30, 2018
Effective date (End): May 29, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Emanuel Carrilho
Grantee:Weliton Pedro Batiston
Supervisor abroad: John Yates III
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Local de pesquisa : Scripps Research Institute, San Diego, United States  
Associated to the scholarship:15/16025-8 - Multidimensional chromatographic systems (nano-HPLC and microchip) hyphenated to tandem mass spectrometry of high resolution for quantitative proteomics, BP.DR


Currently, the analytical performance to characterize all complexity of a proteome is not sufficient. This complexity has been the main subject of study at Yates Lab for the last 25 years with significant scientific impact on the evolution of the field. With this in mind, we propose a series of studies to try to improve analytical strategies to proteomics, such as i) new chemometrics strategies for optimization of hyphenated analytical techniques; ii) a chemical-mathematical model to increase coverage shotgun proteomics; iii) a thermodynamic equation for stop-flow methods on multidimensional liquid chromatography; iv) the development of new nano-columns to top-down proteomics and v) the development of multidimensional separations on µ-chip. Through Yates Lab expertise, we expect to improve our theories using relevant biological questions in the analytical systems developed, as well as, finding solutions to attacking some of the various limitations of bottom-up and top-down proteomics. (AU)