DEVELOPMENT AND APPLICATION OF METABOLOMICS STUDIES BY NMR IN METABOLIC PROFILE OF OBESE PATIENTS UNDERGOING BARIATRIC SURGERY

• Grant number: 12/09318-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2012
• Effective date (End): June 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Anita Jocelyne Marsaioli
• Grantee: Thiago Inácio Barros Lopes
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Metabolomics is the study of organisms or biological systems through its metabolome (all metabolites of an organism) using a combination of high performance analytical methods and multivariate statistical. The metabolomics has emerged rapidly as a new platform for research of biological systems, maximizing the recovery of biological data and complementing other "omics" approaches (as genomics, transcriptomics and proteomics). Currently, metabolomics studies have been used in clinical diagnostic and/or seek by new biomarkers.Despite numerous published articles published in recent years, there is still no consensus about the optimum experimental protocol to metabolomics studies performed by NMR. There is a thin consensus that minimal sample preparation is desirable, though extraction protocols and removal of proteins are often employed in acquisition of spectra. The perfect spectra frequently guide the protocol optimization. However, due to the frequent use of chemometric methods in the interpretation of the results obtained by NMR, we propose this project to optimize the protocol for sample preparation and acquisition parameters and processing of the spectra to obtain the "best chemometric model" in the case concrete.Subsequent studies by NMR metabolomics will be used to assess the changes into metabolic profile resulting from the surgical treatment of obesity. Obesity is a disease characterized by excessive accumulation of body fat, which have been associated comorbidities such diabetes and coronary heart disease. Surgical treatment of obesity is currently indicated for patients with morbid obesity (BMI> 40 kg/m²) and in cases of moderate obesity (BMI> 35 kg/m²) with associated comorbidities. This project going to investigate changes in the metabolome of obese individuals undergoing procedures with Gastric Bypass Roux-en-Y reconstruction and bypass biliopancreatic duct.Finally, we intend to further demonstrate the effectiveness of chemometric methods in the selection of ions in mass spectra for quantification of compounds by GC-MS by selected ion monitoring. The method will be used to select ions for quantification of other compounds of biological importance, such as hydrocarbons and substituted fatty acids.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, THIAGO I. B.; GELONEZE, BRUNO; PAREJA, JOSE C.; CALIXTO, ANTONIO R.; FERREIRA, MARCIA M. C.; MARSAIOLI, ANITA J.. ``Omics{''} Prospective Monitoring of Bariatric Surgery: Roux-En-Y Gastric Bypass Outcomes Using Mixed-Meal Tolerance Test and Time-Resolved H-1 NMR-Based Metabolomics. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, v. 20, n. 7, p. 415-423, JUL 2016. (12/09318-0)

LOPES, THIAGO I. B.; RIBEIRO, MARILENE D. M. M.; MING, CHIU C.; GRIMALDI, RENATO; GONCALVES, LIRENY A. G.; MARSAIOLI, ANITA J.. Comparison of the regiospecific distribution from triacylglycerols after chemical and enzymatic interesterification of high oleic sunflower oil and fully hydrogenated high oleic sunflower oil blend by carbon-13 nuclear magnetic resonance. Food Chemistry, v. 212, p. 641-647, DEC 1 2016. (12/09318-0)

LOPES, THIAGO I. B.; GELONEZE, BRUNO; PAREJA, JOSE C.; CALIXTO, ANTONIO R.; FERREIRA, MARCIA M. C.; MARSAIOLI, ANITA J.. "Omics" Prospective Monitoring of Bariatric Surgery: Roux-En-Y Gastric Bypass Outcomes Using Mixed-Meal Tolerance Test and Time-Resolved H-1 NMR-Based Metabolomics. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, v. 20, n. 7, p. 9-pg., 2016-07-01. (12/09318-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOPES, Thiago Inácio Barros. Applications of nuclear magnetic resonance in the study of: the bariatric surgery, the ethanol production and the regiospecific distribution of triacyclyceride. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.