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| Author(s): |
Thiago Inácio Barros Lopes
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Instituto de Química |
| Defense date: | 2015-08-13 |
| Examining board members: |
Anita Jocelyne Marsaioli;
Luciano Morais Lião;
Luiz Alberto Colnago;
Cláudio Francisco Tormena;
Ljubica Tasic
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| Advisor: | Anita Jocelyne Marsaioli |
| Abstract | |
This thesis shows applications of nuclear magnetic resonance in the study of: bariatric surgery, ethanol and regiospecific distribution triacylglycerides. The first chapter reports main alterations observed in the plasma for ten obese patients 12 months after Roux-en-Y gastric bypass. Several alterations in the metabolic profile as decrease of the glucose, lactate and branched chain amino acids levels; increasing concentrations of ketone bodies, carnitines and p-cresol. Regarding the lipoprotein profile it was observed: decreasing levels of VLDL, LDL and unsaturated lipids; increasing levels of HDL and phosphatidylcholine. Finally, the ratio of unsaturated: saturated fatty acids decreased. In the second chapter, ¹H NMR was applied to the study of ultrasound effect on ethanol production. The PRESAT pulses sequence provided the best quantification results for nine compounds related to fermentation process providing shorter analytic times, when compared to reference methods. The suggested protocol was fully validated in terms of: linearity, LOD, LOQ, precision, accuracy, repeatability and selectivity. However, the application of ultrasound decreased the yield of the fermentation process. In the last part of the work, ¹³C NMR was successfully employed in the study of regiospecific distribution from triacylglycerides obtained by chemical and enzymatic interesterification from sunflower oil and fully hydrogenated sunflower oil blend. In general, chemical interesterification produced a random redistribution of fatty acids, characterized by a random insertion of saturated fatty acids at the sn-2 position of the glycerol moiety. On the other hand, the enzymatic interesterification allowed a partially selective redistribution of fatty acids, maintaining the prevalence of unsaturated fatty acids at the sn-2 position. However, this selectivity decreased at increasing reaction times (AU) | |
| FAPESP's process: | 12/09318-0 - DEVELOPMENT AND APPLICATION OF METABOLOMICS STUDIES BY NMR IN METABOLIC PROFILE OF OBESE PATIENTS UNDERGOING BARIATRIC SURGERY |
| Grantee: | Thiago Inácio Barros Lopes |
| Support Opportunities: | Scholarships in Brazil - Doctorate |