Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Streptozotocin-Induced Maternal Hyperglycemia Increases the Expression of Antioxidant Enzymes and Mast Cell Number in Offspring Rat Ventral Prostate

Texto completo
Autor(es):
Camargo, Ana C. L. ; Dos Santos, Sergio A. A. ; Rinaldi, Jaqueline C. ; Constantino, Flavia B. ; Colombelli, Ketlin T. ; Scarano, Wellerson R. ; Felisbino, Sergio L. ; Justulin, Luis A.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 300, n. 2, p. 291-299, FEB 2017.
Citações Web of Science: 1
Resumo

Gestational diabetes mellitus (GDM) has increased in recent years. Although the cellular and molecular mechanisms involved in GDM-increased risk factors to offspring remained poorly understood, some studies suggested an association between an increase in oxidative stress induced by maternal hyperglycemia and complications for both mothers and newborns. Here, we investigated the impact of maternal hyperglycemia followed by maternal insulin replacement during lactation on the expression of antioxidant enzymes and mast cell number in offspring ventral prostate (VP) at puberty. Pregnant rats were divided into three groups: control (CT); streptozotocin-induced maternal hyperglycemia (MH); and MH plus maternal insulin replacement during lactation (MHI). Male offspring were euthanized at postnatal day (PND) 60 and the VP was removed and processed for histology and Western blotting analyses. Maternal hyperglycemia delayed prostate maturation, and increased mast cell number catalase (CAT), superoxide dismutase (SOD), glutatione-s-transferase (GST-pi), and cyclooxygenase-2 (Cox-2) expression in the offspring of hyperglycemic dams. Maternal insulin replacement restored VP structure, mast cell number and antioxidant protein expression, except for Cox-2, which remained higher in the MHI group. Thus, an increase in oxidative stress induced by intrauterine hyperglycemia impacts prostate development and maturation, which persists until puberty. The overall improvement of maternal metabolism after insulin administration contributes to the restoration of prostate antioxidant enzymes and secretory function. Taken together, our results highlighted that imbalanced physiological maternal-fetal interaction contributes to the impairment of reproductive performance of the offspring from diabetic mothers. Anat Rec, 300:291-299, 2017. (c) 2016 Wiley Periodicals, Inc. (AU)

Processo FAPESP: 11/16522-0 - Efeitos do diabetes gestacional moderado sobre a próstata ventral do rato Wistar: análise morfométrica, proliferação e morte celular e expressão do receptor de andrógeno e da prostateína
Beneficiário:Sérgio Alexandre Alcantara dos Santos
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 13/19673-5 - Efeito do Diabetes gestacional moderado e da reposição materna com insulina sobre a próstata ventral de filhotes machos de rato Wistar: estresse oxidativo e metaloproteinases de matriz
Beneficiário:Ana Carolina Lima Camargo
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 12/18100-9 - Efeitos do diabetes gestacional moderado sobre a próstata ventral do rato Wistar: análise morfométrica, proliferação e morte celular e expressão do receptor de andrógeno e da prostateína
Beneficiário:Luis Antonio Justulin Junior
Linha de fomento: Auxílio à Pesquisa - Regular