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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration

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Autor(es):
Silva, Edmundo ; Reis de Vasconcellos, Luana Marotta ; Rodrigues, Bruno V. M. ; dos Santos, Danilo Martins ; Campana-Filho, Sergio P. ; Marciano, Fernanda Roberta ; Webster, Thomas J. ; Lobo, Anderson Oliveira
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 73, p. 31-39, APR 1 2017.
Citações Web of Science: 12
Resumo

Herein, we developed honeycomb-like scaffolds by combining poly (D, L-lactic acid) (PDLLA) with a high amount of graphene/multi-walled carbon nanotube oxides (MWCNTO-GO, 50% w/w). From pristine multi-walled carbon nanotubes (MWCNT) powders, we produced MWCNTO-GO via oxygen plasma etching (OPE), which promoted their exfoliation and oxidation. Initially, we evaluated PDLLA and PDLLA/MWCNTO-GO scaffolds for tensile strength tests, cell adhesion and cell viability (with osteoblast-like MG-63 cells), alkaline phosphatase (ALP, a marker of osteoblast differentiation) activity and mineralized nodule formation. In vivo tests were carried out using PDLLA and PDLLA/MWCNTO-GO scaffolds as fillers for critical defects in the tibia of rats. MWCNTO-GO loading was responsible for decreasing the tensile strength and elongation-at-break of PDLLA scaffolds, although the high mechanical performance observed (similar to 600 MPa) assures their application in bone tissue regeneration. In vitro results showed that the scaffolds were not cytotoxic and allowed for osteoblast-like cell interactions and the formation of mineralized matrix nodules. Furthermore, MG-63 cells grown on PDLIA/MWCNTO-GO significantly enhanced osteoblast ALP activity compared to controls (cells alone), while the PDLLA group showed similar ALP activity when compared to controls and PDLLA/MWCNTO-GO. Most impressively, in vivo tests suggested that compared to PDLLA scaffolds, PDLLA/MWCNTO-GO had a superior influence on bone cell activity, promoting greater new bone formation. In summary, the results of this study highlighted that this novel scaffold (MWCNTO-GO, 50% w/w) is a promising alternative for bone tissue regeneration and, thus, should be further studied. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 15/08523-8 - Desenvolvimento de um nanosensor fluorescente a partir da eletrofiação de PVA e quantum dots de grafeno: aplicação na detecção de biomarcadores de Alzheimer
Beneficiário:Bruno Vinícius Manzolli Rodrigues
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 15/09697-0 - Eletrofiação de novos nanobiomateriais funcionais à base de peptídeos e polímeros bottlebrush para engenharia tecidual
Beneficiário:Anderson de Oliveira Lobo
Linha de fomento: Bolsas no Exterior - Pesquisa
Processo FAPESP: 16/00575-1 - Biomembranas funcionais a base de peptídeos e polímeros bottlebrush aplicados a engenharia de tecidos
Beneficiário:Fernanda Roberta Marciano
Linha de fomento: Bolsas no Exterior - Pesquisa