Strength training prior to muscle injury potentiates low-level laser therapy (LLLT)-induced muscle regeneration

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Lourenco Morais, Samuel Rodrigues ; Goya, Alexandre Ginei ; Urias, Ursula ; Jannig, Paulo Roberto ; Nova Bacurau, Aline Villa ; Mello, Wagner Garcez ; Faleiros, Paula Lazilha ; Penha Oliveira, Sandra Helena ; Garcia, Valdir Gouveia ; Ervolino, Edilson ; Chakur Brum, Patricia ; Menegati Dornelles, Rita Cassia

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 32, n. 2, p. 317-325, FEB 2017.
• Citações Web of Science: 5

Resumo

We evaluated whether strength training (ST) performed prior to skeletal muscle cryolesion would act as a preconditioning, improving skeletal muscle regeneration and responsiveness to low-level laser therapy (LLLT). Wistar rats were randomly assigned into non-exercised (NE), NE plus muscle lesion (NE + LE), NE + LE plus LLLT (NE + LE + LLLT), strength training (ST), ST + LE, and ST + LE + LLLT. The animals performed 10 weeks of ST (climbing ladder; 3x week; 80% overload). Forty-eight hours after the last ST session, tibialis anterior (TA) cryolesion was induced and LLLT (InGaAlP, 660 nm, 0.035 W, 4.9 J/cm(2)/point, 3 points, spot light 0.028 cm(2), 14 J/cm(2)) initiated and conducted daily for 14 consecutive days. The difference between intergroups was assessed using Student's t test and intragroups by two-way analysis of variance. Cryolesion induced massive muscle degeneration associated with inflammatory infiltrate. Prior ST improved skeletal regeneration 14-days after cryolesion and potentiated the regenerative response to LLLT. Cryolesion induced increased TNF-alpha levels in both NE + LE and ST + LE groups. Both isolated ST and LLLT reduced TNF-alpha to control group levels; however, prior ST potentiated LLLT response. Both isolated ST and LLLT increased IL-10 levels with no additional effect. In contrast, increased TA IL-6 levels were restricted to ST and ST + LE + LLLT groups. TA myogenin mRNA levels were not changed by neither prior ST or ST + LLLT. Both prior ST and LLLT therapies increased MyoD mRNA levels and, interestingly, combined therapies potentiated this response. Myf5 mRNA levels were increased only in ST groups. Taken together, our data provides evidences for prior ST potentiating LLLT efficacy in promoting skeletal muscle regeneration. (AU)

• Processo FAPESP: 13/18907-2 - Influência do estrógeno e do treinamento de força sobre a morfologia e o estresse oxidativo muscular de ratas senis
• Beneficiário: Samuel Rodrigues Lourenço de Morais
• Modalidade de apoio: Bolsas no Brasil - Doutorado