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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Heterologous expression of Streptococcus mutans Cnm in Lactococcus lactis promotes intracellular invasion, adhesion to human cardiac tissues and virulence

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Autor(es):
Freires, Irlan A. ; Aviles-Reyes, Alejandro ; Kitten, Todd ; Simpson-Haidaris, P. J. ; Swartz, Michael ; Knight, Peter A. ; Rosalen, Pedro L. ; Lemos, Jose A. ; Abranches, Jacqueline
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: VIRULENCE; v. 8, n. 1, p. 18-29, 2017.
Citações Web of Science: 10
Resumo

In S. mutans, the expression of the surface glycoprotein Cnm mediates binding to extracellular matrix proteins, endothelial cell invasion and virulence in the Galleria mellonella invertebrate model. To further characterize Cnm as a virulence factor, the cnm gene from S. mutans strain OMZ175 was expressed in the non-pathogenic Lactococcus lactis NZ9800 using a nisin-inducible system. Despite the absence of the machinery necessary for Cnm glycosylation, Western blot and immunofluorescence microscopy analyses demonstrated that Cnm was effectively expressed and translocated to the cell wall of L. lactis. Similar to S. mutans, expression of Cnm in L. lactis enabled robust binding to collagen and laminin, invasion of human coronary artery endothelial cells and increased virulence in G. mellonella. Using an ex vivo human heart tissue colonization model, we showed that Cnm-positive strains of either S. mutans or L. lactis outcompete their Cnm-negative counterparts for tissue colonization. Finally, Cnm expression facilitated L. lactis adhesion and colonization in a rabbit model of infective endocarditis. Collectively, our results provide unequivocal evidence that binding to extracellular matrices mediated by Cnm is an important virulence attribute of S. mutans and confirm the usefulness of the L. lactis heterologous system for further characterization of bacterial virulence factors. (AU)

Processo FAPESP: 13/25080-7 - Prevalência de cepas de Streptococcus mutans expressando Cnm de crianças e adultos e o papel desta glicoproteína nas interações patógeno-hospedeiro
Beneficiário:Irlan de Almeida Freires
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/07231-0 - Prevalência de cepas de Streptococcus mutans expressando Cnm, e o papel desta glicoproteína na ativação plaquetária e aderência a tecidos cardíacos
Beneficiário:Irlan de Almeida Freires
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado