Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms

Autor(es)

Brighenti, Fernanda Lourencao ; Salvador, Marcos Jose ; Lacerda Gontijo, Aline Vidal ; Botazzo Delbem, Alberto Carlos ; Botazzo Delbem, Adina Cleia ; Soares, Cristina Pacheco ; Carvalho de Oliveira, Maria Alcioneia ; Girondi, Camila Miorelli ; Koga-Ito, Cristiane Yumi

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 12, n. 1, p. 15-27, JAN 2017.
• Citações Web of Science: 6

Resumo

Aim: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity. Material \& Methods: Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed. Effects on biofilm inhibition and disruption and cytotoxicity were also evaluated. The most active extract was chemically characterized. Results: Buchenavia tomentosa ethanolic extract showed noticeable antifungal activity and was selected for biofilm experiments. Subinhibitory concentration of extract inhibited fungal adhesion. Maximum killing reached 90% of C. albicans cells in suspension and 65% of cells in biofilms. The active extract was noncytotoxic. Chemical characterization showed the presence of phenols. Ellagic and gallic acids showed activity on C. albicans. Conclusion: B. tomentosa extract and its isolated compound, ellagic acid, presented antibiofilm activity and low toxicity. (AU)

• Processo FAPESP: 08/53299-5 - Identificacao de plantas com propriedades anticariogenicas provenientes do pantanal brasileiro.
• Beneficiário: Cristiane Yumi Koga Ito
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/04114-2 - Identificação de plantas com propriedades anticariogênicas provenientes do Pantanal Brasileiro.
• Beneficiário: Fernanda Lourenção Brighenti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/09666-4 - Implantação de ensaios in vitro para avaliação das características biofarmacêuticas e da bioatividade de substâncias e de produtos bioativos utilizando modelos celulares de candidíase invasiva
• Beneficiário: Aline Vidal Lacerda Gontijo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado