Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Photobiomodulation therapy in the modulation of inflammatory mediators and bradykinin receptors in an experimental model of acute osteoarthritis

Texto completo
Autor(es):
Cavalcante de Oliveira, Vanessa Lima ; Silva, Jr., Jose Antonio ; Serra, Andrey Jorge ; Pallotta, Rodney Capp ; Pereira da Silva, Evela Aparecida ; de Farias Marques, Anna Cristina ; Feliciano, Regiane dos Santos ; Marcos, Rodrigo Labat ; Pinto Leal-Junior, Ernesto Cesar ; Camillo de Carvalho, Paulo de Tarso
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: Lasers in Medical Science; v. 32, n. 1, p. 87-94, JAN 2017.
Citações Web of Science: 7
Resumo

The objective of this study was to evaluate the effects of photobiomodulation therapy (PBMT) on inflammatory indicators, i.e., inflammatory mediators (TNF-alpha and CINC-1), and pain characterized by hyperalgesia and B1 and B2 receptor activation at 6, 24, and 48 h after papain-induced osteoarthritis (OA) in rats. Fifty-four rats were subjected to hyperalgesia evaluations and then divided randomly into three groups-a control group and two groups OA and OA PBMT group by using laser parameters at wavelength (808 nm), output power (50 mW), energy per point (4 Joules), power density (1.78 W/cm(2)), laser beam (0.028 cm(2)), and energy density (144 J/cm(2))-the induction of osteoarthritis was then performed with 20-mu l injections of a 4 % papain solution dissolved in 10 mu l of saline solution, to which 10 mu l of cysteine solution (0.03 M). The statistical analysis was performed using two-way ANOVA with Bonferroni's post hoc test for comparisons between the 6, 24, and 48 h and team points within each group, and between the control, injury, and PBMT groups, and p < 0.05 was considered to indicate a significant difference. The hyperalgesia was evaluated at 6, 24, and 48 h after the injury. PBMT at a wavelength of 808 nm and doses of 4 J, administered afterward, promotes increase at the threshold of pressure stimulus at 6, 24, and 48 h after application and promote cytokine attenuation levels (TNF and CINC-1) and bradykinin receptor (B1 and B2) along the experimental period. We conclude that photobiomodulation therapy was able to promote the reduction of proinflammatory cytokines such as TNF-alpha and CINC-1, to reduce the gene and protein expression of the bradykinin receptor (B1 and B2), as well as increasing the stimulus response threshold of pressure in an experimental model of acute osteoarthritis. (AU)

Processo FAPESP: 15/13677-4 - Efeitos da Laserterapia de Baixa Intensidade na Biodisponibilidade de Células-Tronco Mesenquimais Derivadas de Adipócitos e Implantadas em Joelhos de Ratos Submetidos a Osteoartrite Experimental
Beneficiário:Paulo de Tarso Camillo de Carvalho
Modalidade de apoio: Auxílio à Pesquisa - Regular