Efficient and new method for Tectona grandis in vitro regeneration

Autor(es)

Tambarussi, Evandro V. ; Rogalski, Marcelo ; Galeano, Esteban ; Brondani, Gilvano Ebling ; de Martin, Valentina de Fatima ; da Silva, Lucas Americo ; Carrer, Helaine

• Tipo de documento: Artigo Científico
• Fonte: Crop Breeding and Applied Biotechnology; v. 17, n. 2, p. 124-132, APR 2017.
• Citações Web of Science: 1

Resumo

The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA(3). With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species. (AU)

• Processo FAPESP: 08/00046-2 - Alteração da Biossíntese de ácidos graxos por transformação de cloroplastos
• Beneficiário: Marcelo Rogalski
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/06299-8 - Análise do perfil transcripcional de tecidos lignificados em Tectona grandis L. f
• Beneficiário: Esteban Galeano Gómez
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/05795-0 - Desenvolvimento de metodologias biotecnológicas para micropropagação, regeneração e transformação genética de teca (Tectona grandis L. f.) visando resistência à Hyblaea puera
• Beneficiário: Evandro Vagner Tambarussi
• Modalidade de apoio: Bolsas no Brasil - Mestrado