| Full text | |
| Author(s): |
Tambarussi, Evandro V.
;
Rogalski, Marcelo
;
Galeano, Esteban
;
Brondani, Gilvano Ebling
;
de Martin, Valentina de Fatima
;
da Silva, Lucas Americo
;
Carrer, Helaine
Total Authors: 7
|
| Document type: | Journal article |
| Source: | Crop Breeding and Applied Biotechnology; v. 17, n. 2, p. 124-132, APR 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
The aim of this study was to develop a shoot regeneration protocol for Tectona grandis by examining the effects of pretreatments, regeneration culture medium, adventitious rooting, and acclimatization. The best organogenic potential was achieved when hypocotyls and cotyledonary segments from seedlings germinated on a medium containing TDZ, were cultured for four weeks on MS medium supplemented with BAP/GA(3). With TDZ pretreatment during seed germination, up to 70% of the cotyledonary segments (0.5 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) and 60% of the hypocotyl explants (0.1 TDZ pretreatment and 1.0 BAP + 0.5 GA(3) during regeneration, mg L-1) resulted in shoot regeneration. Finally, 65% of the regenerated shoots rooted, of which 60% were successfully acclimatized ex vitro. Our protocol increases regeneration efficiency for teak and it can serve as a platform for genetic transformation to improve this important tree species. (AU) | |
| FAPESP's process: | 08/00046-2 - Engineering plant fatty acid biosynthesis by chloroplast transformation |
| Grantee: | Marcelo Rogalski |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/06299-8 - Transcriptional profile analysis of lignified tissues in Tectona grandis L. f |
| Grantee: | Esteban Galeano Gómez |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 07/05795-0 - Development of biotechnological methods for micropropagation, regeneration and genetic transformation of teak (Tectona grandis L. f.) to resistance for Hyblaea puera |
| Grantee: | Evandro Vagner Tambarussi |
| Support Opportunities: | Scholarships in Brazil - Master |